Automated High-Throughput Identification and Characterization of Clinically Important Bacteria and Fungi using Rapid Evaporative Ionization Mass Spectrometry
Automated High-Throughput Identification and Characterization of Clinically Important Bacteria and Fungi using Rapid Evaporative Ionization Mass Spectrometry
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DOI：
10.1021/acs.analchem.6b01016
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发表时间：
2016-10-04
影响因子：
7.4
通讯作者：
Takats, Zoltan
中科院分区：
文献类型：
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作者：
Bolt, Frances;Cameron, Simon J. S.;Takats, Zoltan
快速蒸发电离质谱（REIMS）已被证明可以根据微生物的物种特异性脂质谱快速准确地对微生物进行物种鉴定。该小组成员以前的工作表明，使用手持式双极探针允许REIMS直接从培养板分析微生物培养物，而无需任何预先准备。然而，这种分析方法可能不适合高通量临床微生物实验室。在这里，我们报告了一个定制平台的创建，该平台可以实现自动化，高通量REIMS分析，需要最少的用户输入和操作，适用于临床微生物实验室。通过分析从25种微生物物种的不同患者样本中收集的375种不同临床分离株，测试了该高通量平台对临床重要微生物进行物种鉴定的能力。在优化我们的数据分析方法之后，我们在两种REIMS方法之间实现了基本相似的结果。对于手持式双极探针REIMS，实现了96.3%的形态准确度，而对于高通量REIMS，实现了93.9%的准确度。因此，高通量REIMS提供了一种基于质谱的替代方法，用于在临床实验室中快速准确地鉴定临床重要微生物，而无需任何预分析制备步骤。
Rapid evaporative ionization mass spectrometry (REIMS) has been shown to quickly and accurately speciate microorganisms based upon their species-specific lipid profile. Previous work by members of this group showed that the use of a hand-held bipolar probe allowed REIMS to analyze microbial cultures directly from culture plates without any prior preparation. However, this method of analysis would likely be unsuitable for a high-throughput clinical microbiology laboratory. Here, we report the creation of a customized platform that enables automated, high-throughput REIMS analysis that requires minimal user input and operation and is suitable for use in clinical microbiology laboratories. The ability of this high-throughput platform to speciate clinically important microorganisms was tested through the analysis of 375 different clinical isolates collected from distinct patient samples from 25 microbial species. After optimization of our data analysis approach, we achieved substantially similar results between the two REIMS approaches. For hand-held bipolar probe REIMS, a speciation accuracy of 96.3% was achieved, whereas for high-throughput REIMS, an accuracy of 93.9% was achieved. Thus, high-throughput REIMS offers an alternative mass spectrometry based method for the rapid and accurate identification of clinically important microorganisms in clinical laboratories without any preanalysis preparative steps.