Focus on molecules: Smad Interacting Protein 1 (Sip1, ZEB2, ZFHX1B).
Focus on molecules: Smad Interacting Protein 1 (Sip1, ZEB2, ZFHX1B).
复制标题
DOI：
10.1016/j.exer.2010.09.010
复制
发表时间：
2012-08
影响因子：
3.4
通讯作者：
Duncan, Melinda K.
中科院分区：
文献类型：
--
作者：
Grabitz, Abby L.;Duncan, Melinda K.
关键词：
Smad相互作用蛋白1 （Sip1，也被称为ZEB2， ZFHX1B, SMADIP1, FLJ42816, KIAA0569；登录号# NP_055610/人类和# NP_056568/小鼠）是一个1214个氨基酸，140 kDa的蛋白，是双手锌指/同源结构域蛋白ZEB家族的成员。Sip1在脊椎动物中高度保守，在人类和非洲爪蟾之间具有92%的相似性，而与其最近的近亲δ-晶体蛋白增强子结合因子1 （δEF1，也称为ZEB1， ZFHX1A, TCF8， BZP, AREB6, FECD6, NIL2A， ZFHEP, MGC133261）相似度为69%。ZEB转录因子的特征是两个独立的锌指簇，四个在n端（三个CCHH和一个CCHC），三个在c端（所有CCHH）。1A)，两者都必须结合以进行转录调节。同源结构域样区域位于蛋白质中心附近，折叠成具有三个α螺旋的典型结构（PDB: 2DA7），尽管它似乎主要参与蛋白质-蛋白质相互作用。在Sip1和δEF1中也发现了Smad结合域和额外的共抑制因子（如CtBP）和共激活因子（如p300和P/CAF）结合域（Verschueren等人，1999；Vandewalle等人，2009）。Sip1在Lys-391和Lys-866位点被SUMOylated，这降低了其作为转录抑制因子的效力。
Smad Interacting Protein 1 (Sip1, also known as ZEB2, ZFHX1B, SMADIP1, FLJ42816, KIAA0569; accession# NP_055610/human and# NP_056568/mouse) is a 1,214-amino acid, 140 kDa protein which is a member of the ZEB family of two-handed zinc finger/homeodomain proteins. Sip1 is highly conserved in vertebrates, sharing 92% identity between humans and Xenopus, while it is 69% similar to its closest relative, δ-Crystallin Enhancer Binding Factor 1 (δEF1, also known as ZEB1, ZFHX1A, TCF8, BZP, AREB6, FECD6, NIL2A, ZFHEP, MGC133261). ZEB transcription factors are characterized by two separate clusters of zinc fingers, four at the N-terminus (three CCHH and one CCHC) and three at the C-terminus (all CCHH),(Fig. 1A), both of which must bind for transcriptional regulation. The homeodomain-like region, located near the center of the protein, folds into a typical structure with three alpha helices (PDB: 2DA7), although it seems to participate mainly in protein-protein interactions. A Smad binding domain and additional co-repressor (eg CtBP) and co-activator (eg p300 and P/CAF) binding domains are also found in both Sip1 and δEF1 (Verschueren et al., 1999; Vandewalle et al., 2009). Sip1 is SUMOylated at Lys-391 and Lys-866 which reduces its potency as a transcriptional repressor.
影响因子：
1.9
作者：
Zweier, C;Thiel, CT;Rauch, A
通讯作者：
Rauch, A
影响因子：
4.6
作者：
Yoshimoto, A;Saigou, Y;Kondoh, H
通讯作者：
Kondoh, H
影响因子：
4.8
作者：
Verschueren, K;Remacle, JE;Huylebroeck, D
通讯作者：
Huylebroeck, D