Effects of 'hydrophobic mismatch' on the location of transmembrane helices in the ER membrane
Effects of 'hydrophobic mismatch' on the location of transmembrane helices in the ER membrane
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DOI：
10.1016/s0014-5793(01)02415-2
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发表时间：
2001-05-11
期刊：
影响因子：
3.5
通讯作者：
von Heijne, G
中科院分区：
文献类型：
--
作者：
Monné, M;von Heijne, G
我们已经研究了使用糖基化映射方法的多亮氨酸跨膜螺旋（TMH）和ER膜之间的“疏水错配”的影响。我们的研究结果的最简单的解释是，管腔端的TMH位于更深的膜短（负错配）和长（正错配）TMH比多亮氨酸段的中间长度。我们进一步发现，当赖氨酸残基沿着聚亮氨酸延伸移动时，赖氨酸残基对膜中短TMH位置的位置特异性效应随明显的螺旋周期性而变化。我们讨论这些发现的背景下，在疏水性错配的肽-脂质相互作用的模型。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (positive mismatch) TMHs than for poly-Leu segments of intermediate length. We further find that the position-specific effect of Lys residues on the location of short TMHs in the membrane varies with an apparent helical periodicity when the Lys residue is moved along the poly-leu stretch. We discuss these findings in the context of models for peptide-lipid interactions during hydrophobic mismatch. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.