Gene Transfer Targeting Mouse Vestibule Using Adenovirus and Adeno-Associated Virus Vectors
Gene Transfer Targeting Mouse Vestibule Using Adenovirus and Adeno-Associated Virus Vectors
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DOI：
10.1097/mao.0b013e31825368d1
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发表时间：
2012-06-01
影响因子：
2.1
通讯作者：
Ikeda, Katsuhisa
中科院分区：
文献类型：
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作者：
Okada, Hiroko;Iizuka, Takashi;Ikeda, Katsuhisa
假设：本研究评估了如何将基因注射到小鼠前庭中，以及哪种基因是小鼠前庭腺病毒（AdV）载体或腺相关病毒（AAV）载体的最佳基因。近年来有一些关于小鼠前庭基因传递的报道。为了有效地诱导前庭的转基因表达，我们评估了使用各种方法对小鼠内耳进行转染的效率。方法：我们使用半规管方法使用携带绿色荧光蛋白的AdV和AAV（通过泪小管造口术）和圆窗入路。结果：通过小管造口术注射AAV诱导毛细胞、支持细胞前庭器官中的纤维细胞无听觉或平衡功能障碍，提示该方法是最合适的转染方法。结论：经鼻前庭小管造口注射腺相关病毒是一种无创、高效的转染方法，有可能用于人类平衡障碍的修复。
Hypothesis: The present study assessed how to inject a gene into the mouse vestibule and which is the optimum gene to the mouse vestibule adenovirus (AdV) vector or adeno-associated virus (AAV) vector.Background: Loss of vestibular hair cell is seen in various balance disorder diseases. There have been some reports concerning gene delivery to the mouse vestibule in recent years. To effectively induce transgene expression at the vestibule, we assessed the efficiency of inoculating the mouse inner ear using various methods.Methods: We employed an AdV- and AAV-carrying green fluorescent protein using a semicircular canal approach (via a canalostomy) and round window approach.Results: AAV injection via canalostomy induced gene expression at the hair cells, supporting cells, and fibrocytes at the vestibular organs without auditory or balance dysfunction, suggesting it was the most suitable transfection method. This method is thus considered to be a promising strategy to prevent balance dysfunction.Conclusion: AAV injection via canalostomy to the vestibule is the noninvasive and highly efficient transfection method, and this study may have the potential to repair balance disorders in human in the future.