Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes.
Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes.
复制标题
机械力调节心肌细胞中的粘着斑和肋节组装。
DOI：
10.1152/ajpheart.1997.273.2.h546
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Terracio,L
中科院分区：
文献类型：
--
作者：
Sharp,WW;Simpson,DG;Borg,TK;Samarel,AM;Terracio,L
To determine whether the formation and maintenance of focal adhesions and costameres in cardiac myocytes are influenced by the mechanical forces that they transmit, we mechanically unloaded these cells by inhibiting their spontaneous contractile activity with the calcium-channel blocker nifedipine (12 microM). Interference-reflection and fluorescence microscopy revealed that within 24 h of arrest, beta 1-integrin- and vinculin-positive focal adhesions and costameres were disrupted. Loss of mature beta 1-integrin from the cell surface was observed in cell surface-labeling experiments and in Western blots. Subjecting nonbeating cells to a 5% static stretch for 24 h resulted in an increase of 21% for beta 1-integrin and 39% for vinculin. Stretching beating cells resulted in 71 and 9% increases, respectively. Intracellular concentrations of pre-beta 1 were not affected by contractile activity or by stretch. Our results indicate that mechanical forces stabilize the cellular levels of beta 1-integrin and vinculin by possibly regulating their association with the formation and maintenance of focal adhesions and costameres.
