Adult testosterone treatment but not surgical disruption of vomeronasal function augments male-typical sexual behavior in female mice.
Adult testosterone treatment but not surgical disruption of vomeronasal function augments male-typical sexual behavior in female mice.
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DOI：
10.1523/jneurosci.1311-09.2009
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发表时间：
2009-06-17
期刊：
影响因子：
--
通讯作者：
Baum MJ
中科院分区：
文献类型：
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作者：
Martel KL;Baum MJ
最近有报道称，缺乏功能性犁鼻器(VNO)的雌性小鼠表现出典型的雄性性行为，对雌性和雄性同种动物一视同仁。这些结果被引用为表明控制男性典型性行为的回路在两性中都存在，其在女性中的激活被VNO信号调谐地抑制，而不依赖于成人性激素。我们在控制VNO功能被手术破坏的雌性小鼠的内分泌状态的同时，进一步评估了这一假设。在实验1中，与假手术组(VNOi)相比，VNO损毁(VNOx)雌性小鼠对发情雌性或去势、擦拭尿液的雄性小鼠没有表现出更多的上身或骨盆插入行为。当受试者卵巢完整或摘除卵巢并用雌二醇、雌二醇+黄体酮或睾酮治疗时，情况就是这样。在实验2中，接受副嗅球损伤或假损伤的雌性小鼠，在卵巢切除后给予睾酮后，表现出与男性相同的性行为频率。在实验3中，当在卵巢切除后给予睾丸激素时，VNOx和VNOi雌性对发情的雌性或被阉割的雄性表现出相同的男性性行为频率(分别进行测试)。我们的结果证实了早期的报道，即成年睾丸素可以刺激雌性小鼠明显的男性典型性行为。然而，我们未能证实最近的说法，即VNO信号通常抑制控制雌性小鼠典型交配行为表达的神经回路的活动。
It was recently reported that female mice lacking a functional vomeronasal organ (VNO) displayed male-typical sexual behavior indiscriminately towards female and male conspecifics. These results have been cited as showing that a circuit controlling male-typical sex behavior exists in both sexes, with its activation in females being tonically inhibited by VNO signaling, independent of adult sex hormones. We further assessed this hypothesis while controlling the endocrine status of female mice in which VNO function was surgically disrupted. In Experiment 1 VNO lesioned (VNOx) female mice showed no more mounting or pelvic thrusting behavior toward an estrous female or a castrated, urine-swabbed male (presented simultaneously) than sham-operated (VNOi) females. This was true when subjects were either ovary-intact or ovariectomized and treated with estradiol, estradiol + progesterone, or testosterone. In Experiment 2 female mice given accessory olfactory bulb lesions or a sham lesion displayed equivalent frequencies of male sex behaviors when given testosterone following ovariectomy. In Experiment 3 VNOx and VNOi females displayed equivalent frequencies of male sex behaviors towards an estrous female or a castrated male (presented in separate tests), again, when given testosterone following ovariectomy. Our results confirm early reports that adult testosterone can stimulate appreciable male-typical sex behavior in female mice. However, we failed to corroborate the recent claim that VNO signaling normally inhibits the activity of neural circuitry controlling the expression of male-typical mating behavior by female mice.