Dynorphin immunocytochemical localization in brain and peripheral nervous system: preliminary studies.
Dynorphin immunocytochemical localization in brain and peripheral nervous system: preliminary studies.
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强啡肽免疫细胞化学在大脑和周围神经系统中的定位：初步研究。
DOI：
10.1073/pnas.78.2.1260
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发表时间：
1981
影响因子：
11.1
通讯作者：
Goldstein,A
中科院分区：
文献类型：
--
作者：
Watson,SJ;Akil,H;Ghazarossian,VE;Goldstein,A
用阿片肽强啡肽的特异性抗血清，我们进行了免疫细胞化学研究在大鼠脑和外周的分布。在中枢神经系统中，强啡肽染色阳性的细胞位于视上核，而在下丘脑的室旁核中染色不太好。在脑中检测到很少的阳性纤维，表明抗原性的固定和保存存在问题。在垂体中，前叶和中叶未观察到染色，但后叶中检测到重度染色。在豚鼠中，强啡肽抗血清染色的肾上腺嗜铬细胞。这些细胞的染色可用过量的强啡肽-（1-13）或脑啡肽阻断。放射免疫分析显示肾上腺脑啡肽大量过量，表明强啡肽抗血清与肾上腺髓质脑啡肽之间存在交叉竞争。最后，强啡肽抗血清染色豚鼠回肠中的纤维复合物。这些纤维的染色可被中等量的脑啡肽以及较少量的强啡肽-（1-13）阻断。我们的结论是，在某些地方（脑和垂体）强啡肽存在单独的亮氨酸脑啡肽。在脑的其他部位和外周，强啡肽和脑啡肽之间的关系非常复杂，需要进一步研究和改进抗血清。
Using antisera specific for the opioid peptide dynorphin, we have carried out immunocytochemical studies of the distribution in rat brain and periphery. In the central nervous system, cells that stain positively for dynorphin are found in the supraoptic nucleus, with less-well-stained cells in the paraventricular nucleus of the hypothalamus. Few positive fibers were detected in brain, suggesting problems with fixation and preservation of antigenicity. In pituitary no staining was seen in the anterior and intermediate lobes but heavy staining was detected in the posterior lobe. In the guinea pig, adrenal chromaffin cells stained with dynorphin antisera. Staining of these cells could be blocked with excess of dynorphin-(1-13) or either enkephalin. Radioimmunoassays revealed a great excess of the enkephalins in the adrenal, suggesting cross competition between dynorphin antiserum and adrenal medullary enkephalin. Finally, the dynorphin antiserum stained a complex of fibers in guinea pig ileum. Staining of these fibers could be blocked by moderate amounts of enkephalin as well as by smaller amounts of dynorphin-(1-13). We conclude that in some places (brain and pituitary) dynorphin exists separately from leucine-enkephalin. In other parts of brain and in the periphery the relationship between dynorphin and the enkephalins is very complex and requires further study and improved antisera.