Atypical serum transferrin isoform distribution in liver cirrhosis studied by HPLC, capillary electrophoresis and transferrin genotyping
Atypical serum transferrin isoform distribution in liver cirrhosis studied by HPLC, capillary electrophoresis and transferrin genotyping
复制标题
DOI：
10.1016/j.cca.2008.03.033
复制
发表时间：
2008-08-01
影响因子：
5
通讯作者：
Wielders, Jos P. M.
中科院分区：
文献类型：
--
作者：
Arndt, Torsten;van der Meijden, Brenda B.;Wielders, Jos P. M.
背景：二唾液酸转铁蛋白 (CDT) 和三唾液酸转铁蛋白（一种非 CDT 亚型）的不完全分离可能会导致酒精滥用测试中出现假阳性 CDT 结果。我们描述了一种目前未知的二唾液酸转铁蛋白-三唾液酸转铁蛋白桥接现象（二三桥），该现象在肝硬化患者的血清中出现率较高。方法：通过候选参考 CDT HPLC 方法、毛细管电泳（Capillarys-CDT、Sebia）和通过转铁蛋白基因分型。通过电话访谈评估患者的临床背景。 结果：在 HPLC 和 CE 分析中显示二三桥（和三唾液酸转铁蛋白分数增加）的 21 个连续血清样本中，19 个来自有肝硬化病史的患者。基因分型（如果适用，根据 DNA 的可用性：n = 12）产生最常见的纯合转铁蛋白 C1 (6x)，证明二三桥不能用这些样本中的遗传转铁蛋白变异来解释。发现的其他基因型为 C2 (1x)、C1C2 (4x)、C1C3 (1x)。 结论：肝硬化患者转铁蛋白 HPLC 分析中常见的二-三桥现象不能用遗传转铁蛋白变异或三唾液酸转铁蛋白分数增加来解释。尽管需要进一步的研究来评估这种现象与肝硬化之间的关系，但我们的观察可能有助于开发肝硬化的生物标志物。 (C) 2008 Elsevier B.V. 保留所有权利。
Background: An incomplete separation of disialotransferrin (CDT) and trisialotransferrin (a non-CDT isoform) may cause false-positive CDT results in alcohol abuse testing. We describe a currently unknown disialotransferrin-trisialotransferrin-bridging phenomenon (di-tri-bridge) appearing with high prevalence in serum from liver cirrhosis patients.Methods: Twenty one consecutive serum samples with a di-tri-bridge encountered in routine CDT HPLC (Clin-Rep (R)-CDT-on-line, Recipe) were investigated by a candidate reference CDT HPLC method, by capillary electrophoresis (Capillarys-CDT, Sebia) and by transferrin genotyping. Patients clinical background was assessed by telephone interview.Results: Out of 21 consecutive serum samples showing a di-tri-bridge (and increased trisialotransferrin fractions) in HPLC as well as in CE analysis, 19 were from patients with a liver cirrhosis history. Genotyping (where applicable by the availability of DNA: n = 12) yielded most frequently homozygous transferrin C1 (6x), proving that the di-tri-bridge cannot be explained by genetic transferrin variants in these samples. Other genotypes found were C2 (1x), C1C2 (4x), C1C3 (1x).Conclusion: The frequently seen di-tri-bridging phenomenon in transferrin HPLC analysis for patients with liver cirrhosis is not explained by genetic transferrin variants or by an increased trisialotransferrin fraction. Although further studies are needed to assess the relationship between this phenomenon and liver cirrhosis, our observation could be helpful in development of a biomarker for liver cirrhosis. (C) 2008 Elsevier B.V. All rights reserved.