Characteristics of dopamine and GABA transport in primary cultures of astroglial cells.
Characteristics of dopamine and GABA transport in primary cultures of astroglial cells.
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星形胶质细胞原代培养物中多巴胺和 GABA 转运的特征。
DOI：
10.1111/j.1748-1716.1984.tb07464.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Å. Sellström
中科院分区：
文献类型：
--
作者：
E. Hansson;H. Isacsson;Å. Sellström
原代培养的星形胶质细胞来源于新生大鼠大脑半球。对7日龄和14日龄的培养物积累放射性GABA和多巴胺的能力进行了分析。GABA呈集中高亲和力摄取，呈Na+依赖性，Km和Vmax随培养年龄段变化不明显。未观察到依赖于钠离子的多巴胺高亲和力转运。去除胎牛血清后加入0.1 mM的二丁酰环-3‘-5’-腺苷一磷酸(dB-cAMP)，可降低~3H-GABA摄取的Vmax和Km。此药对~3H-GABA和~3H-多巴胺的离子依赖性无明显影响。这一结果很难用细胞分化增强的角度来解释。
Primary astroglial cultures were grown from newborn rat cerebral hemispheres. Cultures, aged 7 and 14 days, were analysed with respect to their capacity to accumulate radioactive GABA and dopamine. Concentrative high-affinity uptake, showing Na+-dependence was observed for GABA where Km and Vmax were not significantly altered with age of the culture. No Na+-dependent high-affinity transport of dopamine was observed. The addition of 0.1 mM dibutyryl-cyclic-3'-5'-adenosinemonophosphate (dB-cAMP) after removal of fetal calf-serum reduced the Vmax as well as the Km of 3H-GABA uptake. The ionic dependence of 3H-GABA and 3H-dopamine was not significantly altered following this treatment. The results are difficult to interpret in terms of a potentiated differentiation of the cells.