2D COSY H-1-NMR - A NEW TOOL FOR STUDYING INSITU BRAIN METABOLISM IN THE LIVING ANIMAL
2D COSY H-1-NMR - A NEW TOOL FOR STUDYING INSITU BRAIN METABOLISM IN THE LIVING ANIMAL
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DOI：
10.1016/0014-5793(90)80247-g
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发表时间：
1990-05-21
期刊：
影响因子：
3.5
通讯作者：
SEYLAZ, J
中科院分区：
文献类型：
--
作者：
BARRERE, B;PERES, M;SEYLAZ, J
带有表面线圈的 2D COSY1H NMR 已用于解析和分配先前已检测到但无法通过常规 ID1H NMR 在活体动物中原位解析或分配的脑代谢物。使用 2D COSY 相关图在整个动物中同时解析和原位分配多种脑代谢物，包括丙氨酸、N-乙酰天冬氨酸、天冬氨酸、胆碱衍生物、肌酸/磷酸肌酸库、GABA、葡萄糖、谷氨酸/谷氨酰胺库、肌醇、乳酸和牛磺酸。整体不可逆缺血导致 2D COSY1H NMR 图谱中交叉峰的出现和消失，对应于丙氨酸、GABA 以及乳酸和葡萄糖消耗的增加。
2D COSY1H NMR with surface coil has been used to resolve and assign cerebral metabolites which had previously been detected but could not be resolved or assigned in situ in the living animal by conventional ID1H NMR. A wide range of cerebral metabolites, including alanine,N-acetyl aspartate, aspartate, choline derivatives, creatine/phosphocreatine pool, GABA, glucose, glutamate/glutamine pool, inositol, lactate and taurine were simultaneously resolved and assigned in situ in the whole animal using the 2D COSY correlation graphs. Global irreversible ischemia caused the appearance and the disappearance of cross-peaks in the 2D COSY1H NMR map, corresponding to increases in alanine, GABA and lactate and glucose depletion.