ALKALI TREATMENT OF MICROROUGH TITANIUM SURFACES AFFECTS MACROPHAGE/MONOCYTE ADHESION, PLATELET ACTIVATION AND ARCHITECTURE OF BLOOD CLOT FORMATION
ALKALI TREATMENT OF MICROROUGH TITANIUM SURFACES AFFECTS MACROPHAGE/MONOCYTE ADHESION, PLATELET ACTIVATION AND ARCHITECTURE OF BLOOD CLOT FORMATION
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DOI：
10.22203/ecm.v021a32
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Hall, Heike
中科院分区：
文献类型：
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作者：
Milleret, Vincent;Tugulu, Stefano;Hall, Heike
钛植入物因其良好的骨整合特性而最常用于骨增量和置换。在这里，超亲水喷砂和酸蚀刻（SBA）钛表面产生的碱处理和他们的反应，部分肝素化的人全血进行了分析。对血凝块形成、血小板活化和补体系统活化进行了分析，揭示了血液和材料表面之间的暴露时间至关重要，因为增加暴露时间会导致更高量的活化血小板、形成更多的血凝块和更强的补体活化。相比之下，与未处理的SBA Ti表面相比，碱处理表面上发现的巨噬细胞/单核细胞数量显著减少。有趣的是，当将未处理的SBA Ti表面与改性的SBA Ti表面进行比较时，在其表面上形成了非常不同的血凝块。在未经处理的钛表面上，血凝块仍然很薄（低于15 mm），片状和非结构化，缺乏大的纤维蛋白纤维网络，而分化表面上的血凝块则聚集在厚度超过30 mm的有组织和分层结构中。在材料表面附近，大多数有核细胞粘附，上面大量的无核血小板保持截留在致密的纤维蛋白纤维网络内，提供整个表面的连续覆盖。这些结果可能表明，结合先前体内研究的结果，证明碱处理的SBA Ti表面在骨整合方面表现更好，面向血液的表面上的连续和结构化的血液成分层支持骨内植入物的后期组织整合。
Titanium implants are most commonly used for bone augmentation and replacement due to their favorable osseointegration properties. Here, hyperhydrophilic sand-blasted and acid-etched (SBA) titanium surfaces were produced by alkali treatment and their responses to partially heparinized whole human blood were analyzed. Blood clot formation, platelet activation and activation of the complement system was analyzed revealing that exposure time between blood and the material surface is crucial as increasing exposure time results in higher amount of activated platelets, more blood clots formed and stronger complement activation. In contrast, the number of macrophages/monocytes found on alkali-treated surfaces was significantly reduced as compared to untreated SBA Ti surfaces. Interestingly, when comparing untreated to modified SBA Ti surfaces very different blood clots formed on their surfaces. On untreated Ti surfaces blood clots remain thin (below 15 mm), patchy and non-structured lacking large fibrin fiber networks whereas blood clots on differentiated surfaces assemble in an organized and layered architecture of more than 30 mm thickness. Close to the material surface most nucleated cells adhere, above large amounts of non-nucleated platelets remain entrapped within a dense fibrin fiber network providing a continuous cover of the entire surface. These findings might indicate that, combined with findings of previous in vivo studies demonstrating that alkali-treated SBA Ti surfaces perform better in terms of osseointegration, a continuous and structured layer of blood components on the blood-facing surface supports later tissue integration of an endosseous implant.