Selective staining by vital dyes of Müller glial cells in retinal wholemounts
Selective staining by vital dyes of Müller glial cells in retinal wholemounts
复制标题
视网膜整体中 Müller 胶质细胞的活体染料选择性染色
作者：
Ortrud Uckermann;I. Iandiev;M. Francke;K. Franze;J. Grosche;S. Wolf;L. Kohen;P. Wiedemann;A. Reichenbach;A. Bringmann
视网膜内的米勒神经胶质细胞可能会对不同的信号分子做出反应，导致细胞内游离钙含量升高。为了证明记录的钙反应在急性分离的视网膜整体中的穆勒细胞中的定位，在钙成像记录完成后，将成年动物和人类的视网膜片暴露于不同的活体染料中。 Mitotracker Orange、Mitotracker Green、Celltracker Orange、Celltracker Green 和一氯二苯甲烷等染料均被 Müller 胶质细胞选择性吸收，而神经元细胞则基本上没有这些染料。通过使用这种方法，可以证明整体中的游离钙变化确实发生在穆勒细胞内。此外，可以在视网膜整体中测量（填充染料的）穆勒神经胶质细胞体以及（无染料）神经元细胞体的横截面积，并且可以确定两种类型细胞的空间密度。穆勒细胞的活性染料负载可能有助于研究刺激引起的视网膜胶质细胞生理学和形态学的改变。 © 2003 Wiley-Liss, Inc.
Müller glial cells within the retina may respond to different signaling molecules with an elevation of their intracellular free calcium. To prove the localization of the recorded calcium responses in Müller cells within acutely isolated retinal wholemounts, retinal pieces from adult animals and humans were exposed to different vital dyes just after the calcium imaging records were finished. The dyes, Mitotracker Orange, Mitotracker Green, Celltracker Orange, Celltracker Green, and monochlorobimane, are all selectively taken up by Müller glial cells, while neuronal cells remain largely devoid of the dyes. By using this method, it can be demonstrated that the free calcium alterations within the wholemounts indeed occur within Müller cells. Moreover, the cross‐sectional areas of (dye‐filled) Müller glial cell bodies, as well as of (dye‐free) neuronal cell bodies, can be measured in retinal wholemounts, and the spatial densities of both types of cells can be determined. The vital dye loading of Müller cells may facilitate investigations of stimulus‐induced alterations of retinal glial cell physiology and morphology. © 2003 Wiley‐Liss, Inc.
影响因子：
2.9
作者：
FERNANDEZCHECA, JC;KAPLOWITZ, N
通讯作者：
KAPLOWITZ, N