Active Hexose Correlated Compound Has Protective Effects in Ischemia?Reperfusion Injury of the Rat Small Intestine
Active Hexose Correlated Compound Has Protective Effects in Ischemia?Reperfusion Injury of the Rat Small Intestine
复制标题
活性己糖相关化合物对大鼠小肠缺血再灌注损伤的保护作用
DOI：
10.1016/j.jss.2019.05.047
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发表时间：
2019
影响因子：
2.2
通讯作者：
Okumura Tadayoshi
中科院分区：
文献类型：
--
作者：
Ueyama Yosuke;Tokuhara Katsuji;Miki Hirokazu;Nakatake Richi;Sakaguchi Tatsuma;Nishizawa Mikio;Kaibori Masaki;Okumura Tadayoshi
背景小肠缺血再灌注损伤是腹主动脉瘤手术或小肠移植中的一个严重问题。活性己糖相关化合物(AHCC)是补充和替代医学中常用的抗炎药物。方法大鼠随机给予正常饲料(IR组)或正常饲料加2%AHCC(IR+AHCC组)10d，禁食1d后夹闭肠系膜上动脉45min。再灌流后1~6h采集肠道和血液标本。结果与IR组比较，IR+AHCC组再灌注6h后，肠粘膜磨损明显减轻，黏膜厚度明显增加。AHCC可降低小肠粘膜诱导型一氧化氮合酶(INOS)、细胞因子诱导的中性粒细胞趋化因子1和白细胞介素6的mRNA表达。AHCC也降低了iNOS蛋白的表达。IR+AHCC组大鼠血清细胞因子诱导的中性粒细胞趋化因子1和肿瘤坏死因子α水平较IR组降低。黏膜核提取液的凝胶迁移率改变分析显示，AHCC抑制了核因子kappaB的激活，并抑制了iNOS反义转录物的表达，从而稳定了iNOSmRNA.结论本研究结果提示AHCC通过抑制核因子kappaB的激活而减少炎性介质的表达。AHCC对肠IR损伤患者可能具有抗炎作用。
BackgroundIschemia–reperfusion (IR) injury of the small intestine is a serious problem in abdominal aortic aneurysm surgery or small intestine transplantation. Active hexose correlated compound (AHCC) is a popular anti-inflammatory drug in complementary and alternative medicine. The aim of this study was to examine whether pretreatment with AHCC reduces intestinal IR injury.MethodsRats were given a normal diet (IR group) or normal diet supplemented with 2% AHCC (IR + AHCC group)ad libitumfor 10 d. After 1 d of fasting, the superior mesenteric artery was occluded by clipping for 45 min. Intestinal and blood samples were collected for 1-6 h after reperfusion. The messenger RNA (mRNA) and protein levels of inflammatory factors were analyzed.ResultsThe IR + AHCC group had reduced mucosal abrasion and significantly increased mucosal thickness of the intestinal tissues 6 h after reperfusion, compared with the IR group. AHCC decreased mRNA expression of inducible nitric oxide synthase (iNOS), cytokine-induced neutrophil chemoattractant 1 and interleukin 6 in the mucosa of the small intestine. AHCC also decreased expression of iNOS protein. Serum levels of cytokine-induced neutrophil chemoattractant 1 and tumor necrosis factor α were decreased in the IR + AHCC group compared with the IR group. Electrophoretic mobility shift assay of mucosal nuclear extracts revealed that AHCC inhibited the activation of nuclear factor kappa B. AHCC also inhibited the expression of iNOS antisense transcript, which stabilizes iNOS mRNA.ConclusionsOur findings suggest that AHCC reduces expression of inflammatory mediators, in part, by inhibiting nuclear factor kappa B activation. AHCC may have anti-inflammatory effect in patients with intestinal IR injury.