Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype.
Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype.
复制标题
DNA 分型方法在白色念珠菌流行病学及其与表型相关性中的应用。
DOI：
10.1093/clinids/12.2.258
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Scherer,S
中科院分区：
文献类型：
--
作者：
Stevens,DA;Odds,FC;Scherer,S
应用的方法ofCandida albicansDNA片段长度多态性分析的相关性与表型和问题的流行病学的念珠菌。到目前为止，在两大类中的29种不同的DNA类型已在91个分离株中得到证实。一种类型，IA 2，是最常见的（41%的分离株）。如小鼠模型中所示，A型在体内多代后是恒定的。来自各个医疗中心的分离株具有多态性，DNA类型在地理上分布广泛。人殖民地和/或有疾病由于C。白念珠菌在两个或两个以上的身体部位几乎总是具有相同的个体类型。性伴侣在生殖器分离株中具有相同的类型。既没有紧密的一致性，也没有随机关联的DNA类型与先前描述的表型组。同样，一些表型特征与DNA类型显著相关。泌尿生殖道分离株与DNA类型IA 2显著相关。定殖和入侵菌株包括许多不同的和重叠的DNA类型。一家医院的一组病例，以前报告是由一种微生物引起的（基于表型研究），现在被证明是由多种类型引起的。这些研究表明，表型转换与从定植到入侵的转变有关。本系列研究展示了DNA分型方法在分类和临床问题中的应用。
The application of methods ofCandida albicansDNA fragment length polymorphism analysis to correlations with phenotype and to questions in the epidemiology ofCandidais shown. Twenty-nine different DNA types, in two broad classes, have thus far been demonstrated in 91 isolates. One type, IA2, was easily the most common (41% of isolates). A type is constant after multiple generations in vivo, as demonstrated in a mouse model. Isolates from individual medical centers were polymorphic, and DNA types were widely distributed geographically. Persons colonized with and/or having disease due toC. albicansat two or more body sites almost always had the same individual type. Sex partners studied had the same type in genital isolates. There was neither tight concordance nor random association of DNA type with previously described phenotypic groups. Likewise, some phenotypic characteristics were significantly associated with DNA type. Urogenital isolates were significantly associated with DNA type IA2. Colonizing and invading isolates included many different and overlapping DNA types. A cluster of cases in a hospital, previously reported to have been caused by a single organism (based on phenotypic studies), was shown to be due to multiple types. Phenotype switching, associated with transition from colonization to invasion, is suggested in these studies. The present series of studies demonstrates the application of DNA typing methods to classification and clinical problems.