Astaxanthin Improves Human Sperm Capacitation by Inducing Lyn Displacement and Activation.
Astaxanthin Improves Human Sperm Capacitation by Inducing Lyn Displacement and Activation.
复制标题
DOI：
10.3390/md13095533
复制
发表时间：
2015-08-25
期刊：
影响因子：
5.4
通讯作者：
Bordin L
中科院分区：
文献类型：
--
作者：
Andrisani A;Donà G;Tibaldi E;Brunati AM;Sabbadin C;Armanini D;Alvisi G;Gizzo S;Ambrosini G;Ragazzi E;Bordin L
虾青素（Asta）是类胡萝卜素家族中的一种光保护红色色素，因其多种有益特性而闻名。在这项研究中，评估了亚洲对分离的人类精子的影响。获能过程包括一系列转化，以使精子获得潜在的卵母细胞受精的正确特征，包括产生一定量的活性氧（ROS），精子外膜的胆固醇消耗以及头部区域的蛋白酪氨酸磷酸化（tyrp）过程。精子图值正常的志愿者根据其产生正确内源性ROS含量的能力分为两组。分析患者组（PG）和对照组（CG）在存在或不存在Asta的情况下，酪氨酸磷酸化（tyrp）模式和顶体反应细胞（ARC）和非活细胞（NVC）的百分比。此外，研究人员还研究了ROS对膜重组的参与以及与脂筏相关的Src家族激酶Lyn的存在。结果表明，Lyn存在于人类精子的膜中，在静息状态下主要局限在中间。在获能后，Lyn在筏子迁移的同时转移到头部，从而允许头部蛋白的tyrp。亚洲成功地触发了PG精子的Lyn易位，从而绕过了受损的ros相关的筏和Lyn易位机制。在这项研究中，我们发现ROS的产生与脂筏和Lyn重新定位之间存在相互依赖关系，导致细胞发生连续的顶体反应（AR）。亚洲，通过改善PG精子功能，可用于减少男性特发性不育症。
Astaxanthin (Asta), a photo-protective red pigment of the carotenoid family, is known for its multiple beneficial properties. In this study, the effects of Asta on isolated human sperm were evaluated. Capacitation involves a series of transformations to let sperm acquire the correct features for potential oocyte fertilization, including the generation of a controlled amount of reactive oxygen species (ROS), cholesterol depletion of the sperm outer membrane, and protein tyrosine phosphorylation (Tyr-P) process in the head region. Volunteers, with normal spermiogram values, were divided in two separate groups on the basis of their ability to generate the correct content of endogenous ROS. Both patient group (PG) and control group (CG) were analysed for Tyr-phosphorylation (Tyr-P) pattern and percentages of acrosome-reacted cells (ARC) and non-viable cells (NVC), in the presence or absence of Asta. In addition, the involvement of ROS on membrane reorganization and the presence of Lyn, a Src family kinase associated with lipid rafts, were investigated. Results show that Lyn is present in the membranes of human sperm, mainly confined in midpiece in resting conditions. Following capacitation, Lyn translocated to the head concomitantly with raft relocation, thus allowing the Tyr-P of head proteins. Asta succeeded to trigger Lyn translocation in PG sperm thus bypassing the impaired ROS-related mechanism for rafts and Lyn translocation. In this study, we showed an interdependence between ROS generation and lipid rafts and Lyn relocation leading the cells to undergo the successive acrosome reaction (AR). Asta, by ameliorating PG sperm functioning, may be utilised to decrease male idiopathic infertility.