ADAPTATIONAL CHANGES OF FATTY-ACID COMPOSITION AND THE PHYSICAL STATE OF MEMBRANE-LIPIDS FOLLOWING THE CHANGE OF GROWTH TEMPERATURE IN YERSINIA-ENTEROCOLITICA
ADAPTATIONAL CHANGES OF FATTY-ACID COMPOSITION AND THE PHYSICAL STATE OF MEMBRANE-LIPIDS FOLLOWING THE CHANGE OF GROWTH TEMPERATURE IN YERSINIA-ENTEROCOLITICA
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DOI：
10.1111/j.1348-0421.1991.tb01630.x
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发表时间：
1991-01-01
影响因子：
2.6
通讯作者：
KANEMASA, Y
中科院分区：
文献类型：
--
作者：
NAGAMACHI, E;SHIBUYA, S;KANEMASA, Y
小肠结肠炎耶尔森氏菌能够在 4 至 45°C 的广泛温度范围内生长。这种生物体如何响应生长温度的变化而改变其膜脂非常有趣。分析了在5℃、15℃、25℃和37℃下培养的细胞膜脂的脂肪酸，并表征了这些膜脂的物理状态。该细菌的主要磷脂是磷脂酰乙醇胺、磷脂酰甘油、心磷脂、溶血磷脂酰甘油和溶血磷脂酰乙醇胺。没有观察到磷脂组成对培养温度的显着差异。我们之前的论文报道小肠结肠炎耶尔森氏菌膜磷脂的主要脂肪酸为C15:0、C16:0、C16:1、环丙烷C17:0和C18:0。然而，随着培养温度的变化，观察到脂肪酸组成的一些差异。当培养温度升高时，饱和脂肪酸和环丙烷脂肪酸显着增加，不饱和脂肪酸减少。当培养温度降低时观察到相反的现象。从膜物理状态的角度，使用尼龙微胶囊方法分析适应性变化。在每个培养温度下生长的细胞的膜脂的相变发生在低于培养温度约5℃和高于培养温度约10℃的范围内。因此，认为小肠结肠炎耶尔森氏菌通过改变膜脂肪酸组成来响应生长温度而维持其膜刚性和流动性。
Yersinia enterocolitica is capable of growing in a broad range of temperatures from 4 to 45 C. How this organism alters its membrane lipids in response to the change of growth temperature is very interesting. The fatty acids of membrane lipids of cells cultured at 5, 15, 25 and 37 C were analyzed and the physical states of these membrane lipids were characterized. The major phospholipids of this bacterium were phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, lysophosphatidylglycerol and lysophosphatidylethanolamine. No significant difference in phospholipid composition in response to culture temperatures was observed. It was reported in our previous paper that the major fatty acids of membrane phospholipids of Y. enterocolitica were C15:0, C16:0, C16:1, cyclopropane C17:0 and C18:0. Some differences in the fatty acid composition were, however, observed with the change of culture temperature. When the culture temperature was raised, the saturated and cyclopropane fatty acids substantially increased and the unsaturated ones decreased. A reverse phenomenon was observed when culture temperature was lowered. From the viewpoints of membrane physical state, adaptational changes were analyzed using a nylon microcapsule method. Phase transition in membrane lipids of cells grown at each culture temperature took place in the range of about 5 C below and about 10 C above the culture temperature. It is, therefore, considered that Y. enterocolitica maintains its membrane rigidity and fluidity in response to growth temperature by changing the membrane fatty acid composition.