RAPID INDUCTION OF POLYADENYLATED H1-HISTONE MESSENGER-RNAS IN MOUSE ERYTHROLEUKEMIA-CELLS IS REGULATED BY C-MYC
RAPID INDUCTION OF POLYADENYLATED H1-HISTONE MESSENGER-RNAS IN MOUSE ERYTHROLEUKEMIA-CELLS IS REGULATED BY C-MYC
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DOI：
10.1128/mcb.9.6.2332
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发表时间：
1989-06-01
影响因子：
5.3
通讯作者：
SKOULTCHI, AI
中科院分区：
文献类型：
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作者：
CHENG, GH;SKOULTCHI, AI
小鼠红白血病细胞的化学诱导分化是一个多步骤的过程，包括一个预定型期，其中暴露于诱导剂导致细胞不可逆地定型为终末分化。细胞原癌基因表达的某些变化是预定型阶段的一个重要特征。我们已经确定了两个H1组蛋白基因，在此期间迅速诱导。与大多数组蛋白基因不同，这两个H1基因编码具有长3“非翻译区的多聚腺苷酸化mRNA。为了研究H1 mRNA的诱导和原癌基因表达变化之间的关系，我们研究了两个独立的系列小鼠红白血病细胞系，这些细胞系由于c-myc或c-myb转染拷贝的表达失调而被抑制分化。结果表明，H1 mRNA的诱导受c-myc负调控。两个H1组蛋白基因是由c-myc调控的特异性细胞基因的第一个例子。他们的诱导时间表明，他们可能发挥重要作用，在实现承诺的终端分化。
Chemically induced differentiation of murine erythroleukemia cells is a multistep process involving a precommitment period in which exposure to inducer leads to cells that are irreversibly committed to terminal differentiation. Certain changes in the expression of cellular proto-oncogenes are an important feature of the precommitment phase. We have identified two H1 histone genes that are rapidly induced during this period. Unlike most histone genes, these two H1 genes encode polyadenylated mRNAs with long 3'' untranslated regions. To investigate the relationship between induction of the H1 mRNAs and changes in proto-oncogene expression, we studied two independent series of mouse erythroleukemia cell lines that are inhibited from differentiating because of deregulated expression of transfected copies of c-myc or c-myb. The results showed that induction of the H1 mRNAs was negatively regulated by c-myc. The two H1 histone genes are among the first examples of specific cellular genes that are regulated by c-myc. The timing of their induction suggests that they may play an important role in achieving commitment to terminal differentiation.