Comparison of the Abbott 7000 and Bayer 340 systems for measurement of hepatitis C virus load
Comparison of the Abbott 7000 and Bayer 340 systems for measurement of hepatitis C virus load
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DOI：
10.1128/jcm.00202-07
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发表时间：
2007-09-01
影响因子：
9.4
通讯作者：
Dewar, Robin L.
中科院分区：
文献类型：
--
作者：
Highbarger, Helene C.;Hu, Zonghui;Dewar, Robin L.
本研究比较了两种用于测量丙型肝炎病毒（HCV）RNA水平的市售检测试剂盒，即Bayer HCV RNA（版本3.0）分支DNA检测试剂盒和Abbott HCV分析物特异性试剂实时PCR检测试剂盒，以评估其定量关系、性能简易性和完成时间。研究组包括从NIAID人类免疫缺陷病毒（HIV）门诊随机选择的HIV 1型和HCV感染患者。来自66例接受不同方案治疗的HIV和HCV共感染患者的184份样本进行了分析，以获得结果的相关性和一致性。结果表明，这两种测定法在重叠的线性范围内相关性良好，并显示出良好的一致性。根据获得的结果，我们推导出了一个数学公式，用于比较两种检测试剂盒之间的病毒载量结果，其表示为log（10）Abbott检测试剂盒测量值= 0.032 + 1.01 log（10）Bayer检测试剂盒测量值。尽管在患者治疗的整个过程中优选使用相同的定量测定，但可以在重叠线性范围（615至7，700，000 IU/ml）内对通过这些测定中的任一种获得的结果进行HCV RNA水平的有效比较。
This study compared two commercially available assays for the measurement of hepatitis C virus (HCV) RNA levels, the Bayer HCV RNA (version 3.0) branched DNA assay and the Abbott HCV anallyte-specific reagent real-time PCR assay, to assess their quantitative relationships, ease of performance, and time to completion. The study group consisted of randomly selected patients from the NIAID human immunodeficiency virus (HIV) outpatient clinic who were infected with HIV type 1 and HCV. One hundred eighty-four samples from 66 patients coinfected with HIV and HCV receiving treatments under various protocols were analyzed for the correlation and agreement of the results. The results indicated that the two assays correlate well in the overlapping linear ranges of the assays and show good agreement. From the results obtained, we have derived a mathematical formula to compare the viral load results between the two assays, which is given as log(10) Abbott assay measure = 0.032 + 1.01 log(10) Bayer assay measure. Although it is preferable to use the same quantitation assay throughout the course of a patient's treatment, valid comparisons of the HCV RNA levels may be made between the results obtained by either of these assays in the overlapping linear range (615 to 7,700,000 IU/ml).