A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae.
A comprehensive transcriptomic view of renal function in the malaria vector, Anopheles gambiae.
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DOI：
10.1016/j.ibmb.2015.05.007
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发表时间：
2015-12
影响因子：
3.8
通讯作者：
G. Overend;P. Cabrero;K. Halberg;L. Ranford-Cartwright;D. Woods;S. Davies;J. Dow
中科院分区：
文献类型：
--
作者：
G. Overend;P. Cabrero;K. Halberg;L. Ranford-Cartwright;D. Woods;S. Davies;J. Dow
肾功能对维持体内平衡至关重要。这对于经历完全变态的昆虫特别重要;幼虫蚊子必须在淡水栖息地生存，而成虫是陆地的，成熟的雌性必须在吸血后保持离子和流体的稳态。为了研究成功发育到成年所需的生理适应，我们使用Affyphidae阵列研究了冈比亚按蚊的马氏管转录组。我们评估转录在几种条件下，作为第三龄幼虫，作为成年男性喂养糖，作为成年女性喂养糖，和成年女性血餐后。除了提供迄今为止最详细的转录组学数据的按蚊Malpighian小管，数据提供了独特的信息，从淡水到陆地栖息地的切换所需的肾脏适应，性别差异，花蜜喂养和吸血之间的对比。我们发现了与生活方式，性别和饮食的个体发育变化相关的明显差异，特别是控制液体分泌的神经肽受体，以及影响体积和组成的水和离子转运蛋白。这些数据还与果蝇小管的转录组学相结合，允许对双翅目小管功能的基因进行荟萃分析。为了进一步研究跨物种的肾脏保护，我们选择了四个在按蚊中高度富集的直向同源物的黑腹果蝇基因，并产生了RNAi敲除果蝇。这些基因中有三个被证明是必不可少的，在1.5亿年的系统发育分离中显示出关键功能的保守性。这一广泛的数据集可作为在线资源MozTubules.org获得，并可能被挖掘为新的杀虫剂靶标，这些靶标可以影响这种害虫物种的这一关键器官。
Renal function is essential to maintain homeostasis. This is particularly significant for insects that undergo complete metamorphosis; larval mosquitoes must survive a freshwater habitat whereas adults are terrestrial, and mature females must maintain ion and fluid homeostasis after blood feeding. To investigate the physiological adaptations required for successful development to adulthood, we studied the Malpighian tubule transcriptome of Anophelesgambiaeusing Affymetrix arrays. We assessed transcription under several conditions; as third instar larvae, as adult males fed on sugar, as adult females fed on sugar, and adult females after a blood meal. In addition to providing the most detailed transcriptomic data to date on the Anopheles Malpighian tubules, the data provide unique information on the renal adaptations required for the switch from freshwater to terrestrial habitats, on gender differences, and on the contrast between nectar-feeding and haematophagy. We found clear differences associated with ontogenetic change in lifestyle, gender and diet, particularly in the neuropeptide receptors that control fluid secretion, and the water and ion transporters that impact volume and composition. These data were also combined with transcriptomics from the Drosophila melanogaster tubule, allowing meta-analysis of the genes which underpin tubule function across Diptera. To further investigate renal conservation across species we selected fourD.melanogastergenes with orthologues highly enriched in theAnophelestubules, and generated RNAi knockdown flies. Three of these genes proved essential, showing conservation of critical functions across 150 million years of phylogenetic separation. This extensive data-set is available as an online resource, MozTubules.org, and could potentially be mined for novel insecticide targets that can impact this critical organ in this pest species.