RHIPICEPHALUS-APPENDICULATUS - LARVAL FEEDING SITES IN GUINEA-PIGS ACTIVELY SENSITIZED AND RECEIVING IMMUNE SERUM
RHIPICEPHALUS-APPENDICULATUS - LARVAL FEEDING SITES IN GUINEA-PIGS ACTIVELY SENSITIZED AND RECEIVING IMMUNE SERUM
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DOI：
10.1016/0014-4894(83)90004-8
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发表时间：
1983-01-01
影响因子：
2.1
通讯作者：
ASKENASE, PW
中科院分区：
文献类型：
--
作者：
BROWN, SJ;WORMS, MJ;ASKENASE, PW
幼虫的组织学分析。在蜱附着后6、24、48、72和96小时，在未处理和主动致敏的豚鼠中制作无钩幼虫摄食部位。随着初次进食的进展，蜱虫口器入口处的空腔进入最上层真皮，周围的细胞浸润（病变）都增加。早期（6 h）病变以嗜酸性粒细胞为主（占浸润的46%），24 h（55%）和48 h（68%）以中性粒细胞为主，72 h（44%）再次以嗜酸性粒细胞为主，96 h（78%）以嗜碱性粒细胞为主。在表达获得性抗性的动物的二次喂食部位，病变大小随着蜱虫喂食的进展而增加，并且在每个观察时间至少是初次喂食中观察到的病变大小的两倍。蜱虫口器入口部位的真皮腔偶尔发生，并且尺寸减小，表明蜱虫摄食改变。嗜碱性粒细胞占浸润的61 - 91%。第二种重要的细胞类型是嗜酸性粒细胞，丰度范围为7%至21%。免疫血清的接受者在喂食蜱周围有较小的细胞浸润，但嗜碱性粒细胞也占主导地位。嗜碱性粒细胞似乎是主要的宿主细胞参与获得性抵抗蜱喂养的深刻的皮肤嗜碱性粒细胞反应，其特征在于在主动和被动致敏宿主的蜱幼虫的免疫反应所示。两种宿主蜱虫取食部位的嗜酸性粒细胞显著蓄积的结果表明，这些细胞也可能有助于获得性耐药性。
Histological analyses of larval R. appendiculatus feeding sites in naive and actively sensitized guinea pigs were made at 6, 24, 48, 72, and 96 h post-tick attachment. As primary feedings progressed the cavity at the entrance of the ticks mouthparts into the uppermost dermis, and the surrounding cellular infiltrate (lesion) both increased. Early (6 h) lesions were dominated by eosinophils (46% of the infiltrate), neutrophils predominated at 24 (55%) and 48 h (68%), eosinophils again predominated at 72 h (44%), and finally basophils were dominant at 96 h (78%). At sites of secondary feedings in animals expressing acquired resistance, lesion size increased as tick feeding progressed and at each observation time was at least twice that observed in primary feedings. Dermal cavities at the site of entrance of the ticks mouthparts were occasional in occurrence and were reduced in size indicating altered tick feeding. Basophils were dominant at all observation times ranging from 61 to 91% of the infiltrate. The 2nd cell type of significance was the eosinophil, ranging in abundance from 7 to 21%. Recipients of immune serum had a smaller cellular infiltrate around feeding ticks, but basophils were also dominant. Basophils appear to be the principal host cell involved in acquired resistance to tick feeding as indicated by the profound cutaneous basophil reaction that characterized the immune response to larval ticks both in actively and passively sensitized hosts. The findings of significant eosinophil accumulations at tick feeding sites of both hosts indicates that these cells may also contribute to acquired resistance.