Magnetic resonance imaging: a tool to monitor and optimize enzyme distribution during porcine pancreas distention for islet isolation.
Magnetic resonance imaging: a tool to monitor and optimize enzyme distribution during porcine pancreas distention for islet isolation.
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DOI：
10.1111/xen.12108
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发表时间：
2014-09
影响因子：
3.9
通讯作者：
Papas KK
中科院分区：
文献类型：
--
作者：
Scott WE 3rd;Weegman BP;Balamurugan AN;Ferrer-Fabrega J;Anazawa T;Karatzas T;Jie T;Hammer BE;Matsumoto S;Avgoustiniatos ES;Maynard KS;Sutherland DE;Hering BJ;Papas KK
Porcine islet xenotransplantation is emerging as a potential alternative for allogeneic clinical islet transplantation. Optimization of porcine islet isolation in terms of yield and quality is critical for the success and cost effectiveness of this approach. Incomplete pancreas distension and inhomogeneous enzyme distribution have been identified as key factors for limiting viable islet yield per porcine pancreas. The aim of this study was to explore the utility of Magnetic Resonance Imaging (MRI) as a tool to investigate the homogeneity of enzyme delivery in porcine pancreata. Traditional and novel methods for enzyme delivery aimed at optimizing enzyme distribution were examined. Pancreata were procured from Landrace pigs via en bloc viscerectomy. The main pancreatic duct was then cannulated with an 18g winged catheter and MRI performed at 1.5 T. Images were collected before and after ductal infusion of chilled MRI contrast agent (gadolinium) in physiological saline. Regions of the distal aspect of the splenic lobe and portions of the connecting lobe and bridge exhibited reduced delivery of solution when traditional methods of distension were utilized. Use of alternative methods of delivery (such as selective re-cannulation and distension of identified problem regions) resolved these issues and MRI was successfully utilized as a guide and assessment tool for improved delivery. Current methods of porcine pancreas distension do not consistently deliver enzyme uniformly or adequately to all regions of the pancreas. Novel methods of enzyme delivery should be investigated and implemented for improved enzyme distribution. MRI serves as a valuable tool to visualize and evaluate the efficacy of current and prospective methods of pancreas distension and enzyme delivery.
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影响因子：
0.9
作者：
Sabat, M;Godlewska, E;Orlowski, T
通讯作者：
Orlowski, T
影响因子：
6.2
作者：
Balamurugan AN;Loganathan G;Bellin MD;Wilhelm JJ;Harmon J;Anazawa T;Soltani SM;Radosevich DM;Yuasa T;Tiwari M;Papas KK;McCarthy R;Sutherland DE;Hering BJ
通讯作者：
Hering BJ
影响因子：
3.9
作者：
Dufrane, Denis;D'hoore, William;Gianello, Pierre
通讯作者：
Gianello, Pierre
影响因子：
3.9
作者：
Abrahante, Juan E.;Martins, Kyra;Murtaugh, Michael P.
通讯作者：
Murtaugh, Michael P.
影响因子：
3.9
作者：
Kim, Jae Hyeon;Kim, Hyoung-Il;Park, Kyong Soo
通讯作者：
Park, Kyong Soo