STUDIES ON ADJUVANT-INDUCED POLYARTHRITIS IN RATS .1. ADJUVANT COMPOSITION, ROUTE OF INJECTION, AND REMOVAL OF DEPOT SITE
STUDIES ON ADJUVANT-INDUCED POLYARTHRITIS IN RATS .1. ADJUVANT COMPOSITION, ROUTE OF INJECTION, AND REMOVAL OF DEPOT SITE
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DOI：
10.1002/art.1780050604
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发表时间：
1962-01-01
影响因子：
--
通讯作者：
JONES, RS
中科院分区：
文献类型：
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作者：
WARD, JR;JONES, RS
研究了注射弗氏佐剂的组成、剂量、注射途径和贮库部位反应对大鼠多发性关节炎的影响。皮内注射酪酸肌杆菌在油诱导的关节炎中的频率比皮下或静脉途径高得多。最小有效皮内剂量为0.06 mg M。0.02毫升油中的丁酸; 0.1毫升油中需要0.6毫克分枝杆菌才能使100%的大鼠产生多发性关节炎。分枝杆菌的蜡级分没有诱发关节炎。注射部位的佐剂贮库和炎症都不重要，因为即使在注射后2小时内通过切断尾部以去除注射佐剂的贮库也会发生多发性关节炎。虽然佐剂诱导的多关节炎的机制仍然未知，但关节疾病似乎取决于佐剂的特定传播部位。
The composition and quantity of Freund-type adjuvant injected, the route of injection and reaction at the depot site were studied for their roles in producing polyarthritis in rats. The intradermal injection of Myobacterium butyricum in oil induced arthritis with much higher frequency than the subcutaneous or intravenous route. The minimal effective intradermal dose was 0.06 mg M. butyricum in 0.02 ml oil; 0.6 mg mycobacteria in 0.1 ml oil was required to produce polyarthritis in 100 per cent of rats. Wax fractions of mycobacteria did not induce arthritis. Neither a depot of adjuvant nor inflammation at the injection site was important as polyarthritis developed even through amputation of the tail to remove the depot of injected adjuvant was performed within 2 hours after injection. While the mechanism of adjuvant-induced polyarthritis remains unknown, the joint disease appeared dependent upon specific sites of dissemination of the adjuvant.