The effects of the surface topography of micromachined titanium substrata on cell behavior in vitro and in vivo
The effects of the surface topography of micromachined titanium substrata on cell behavior in vitro and in vivo
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DOI：
10.1115/1.2798042
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发表时间：
1999-02-01
影响因子：
1.7
通讯作者：
Chehroudi, B
中科院分区：
文献类型：
--
作者：
Brunette, DM;Chehroudi, B
表面特性，包括表面形貌和化学特性，在确定组织对生物材料的反应方面是最重要的。微加工技术使精确控制的表面形貌的生产成为可能，这些表面形貌已被用作培养细胞和体内植入设备的衬底。本文综述了组织对植入物反应所涉及的细胞行为的各个方面，并着重介绍了地形的影响。微加工凹槽表面在体外产生上皮细胞的定向和定向运动，并能抑制种植体上皮的向下生长。这种影响取决于凹槽的尺寸，它们被上皮细胞-细胞相互作用所改变。成纤维细胞在凹槽表面表现出类似的接触导向，但体外成纤维细胞的形状与体内发现的明显不同。表面形貌在建立种植体附近的组织结构方面很重要，光滑的表面通常与纤维组织包裹有关。凹槽结构在短期内似乎有希望减少包裹性II，但需要采用三维重建和不同的地形进行更多的研究，以更好地了解种植体附近结缔组织的组织过程。微细加工表面可增加大鼠皮下植入表面附近矿化骨样组织结节的出现频率。这些有凹槽的结节在培养和植入物上都有定向。由于人口密度和微运动等因素的数量和复杂性，细胞在体外和体内微加工底物上的行为很难进行详细比较，这些因素在这些条件下是不同的。
Surface properties, including topography and chemistry, are of prime importance in establishing the response of tissues to biomaterials. Microfabrication techniques have enabled the production of precisely controlled surface topographies that have been used as substrata for cells in culture and on devices implanted in vivo. This article reviews aspects of cell behavior involved in tissue response to implants with an emphasis on the effects of topography. Microfabricated grooved surfaces produce orientation and directed locomotion of epithelial cells in vitro and can inhibit epithelial downgrowth on implants. The effects depend on the groove dimensions and they are modified by epithelial cell-cell interactions. Fibroblasts similarly exhibit contact guidance on grooved surfaces, but fibroblast shape in vitro differs markedly from that found in vivo. Surface topography is important in establishing tissue organization adjacent to implants, with smooth surfaces generally being associated with fibrous tissue encapsulation. Grooved topographies appear to have promise in reducing encapsulation ii in the short term, but additional studies employing three-dimensional reconstruction and diverse topographies are needed to understand better the process of connective-tissue organization adjacent to implants. Microfabricated surfaces can increase the frequency of mineralized bone-like tissue nodules adjacent to subcutaneously implanted surfaces in rats. Orientation of these nodules with grooves occurs both in culture and on implants. Detailed comparisons of cell behavior on micromachined substrata in vitro and in vivo are difficult because of the number and complexity of factors, such as population density and micromotion, that cart differ between these conditions.