CLONING AND SEQUENCING OF GOLDFISH ACTIVIN SUBUNIT GENES - STRONG STRUCTURAL CONSERVATION DURING VERTEBRATE EVOLUTION
CLONING AND SEQUENCING OF GOLDFISH ACTIVIN SUBUNIT GENES - STRONG STRUCTURAL CONSERVATION DURING VERTEBRATE EVOLUTION
复制标题
DOI：
10.1006/bbrc.1993.1683
复制
发表时间：
1993-06-15
影响因子：
3.1
通讯作者：
PETER, RE
中科院分区：
文献类型：
--
作者：
GE, W;GALLIN, WJ;PETER, RE
抑制素和激活素是结构上相关的二聚体肽，具有两个不同但相关的亚基βA和βB。利用聚合酶链反应（PCR）技术，从金鱼基因组DNA中克隆了编码激活素βA和βB成熟肽的基因，并进行了序列测定。结果表明，金鱼βA亚基与人βA亚基的同源性为78%，两种βB亚基与人βB亚基的同源性均在94%以上。这是第一次证明激活素家族的肽在鱼类。
Inhibin and activin are structurally related dimeric peptides sharing two distinct but related subunits, βA and βB. By using polymerase chain reaction (PCR), we have cloned and sequenced activin βA and βB genes encoding the mature region of the peptides from the goldfish genomic DNA. A single form of βA and two forms of βB subunits were found. There is high identity with mammalian counterparts; the goldfish βA subunit has 78% protein sequence identity with human βA subunit, and the two βB subunits have more than 94% sequence identity with human βB subunit. This is the first demonstration of the activin family of peptides in the fishes.