Mycolicibacterium sp. strain PAM1, an alfalfa rhizosphere dweller, catabolizes PAHs and promotes partner-plant growth
Mycolicibacterium sp. strain PAM1, an alfalfa rhizosphere dweller, catabolizes PAHs and promotes partner-plant growth
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DOI：
10.1016/j.micres.2021.126885
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发表时间：
2021-10-05
影响因子：
6.7
通讯作者：
Turkovskaya, Olga, V
中科院分区：
文献类型：
--
作者：
Golubev, Sergey N.;Muratova, Anna Yu.;Turkovskaya, Olga, V
本研究从受石油烃污染的苜蓿根际土壤中分离出一株能降解多环芳烃的分枝杆菌，并对该菌与植物的相互作用进行了初步研究。从我们感兴趣的生态位中分离到的芘降解菌Mycolicibacterium sp. PAM 1可以降解荧蒽、蒽、芴和菲。根据PAM 1对不同PAHs的生长曲线和降解速率，发现污染物的可利用性顺序为菲>芴>荧蒽~芘>蒽。对于每种PAH，鉴定了分解代谢产物。PAM 1具有nidA和nidB功能基因。新的数据建模的2D和3D结构，内在的结构紊乱，和nidA和nidB基因产物的分子动力学。鉴定的芘降解基因和中间体表明PAM 1具有已知分枝杆菌芘降解物特有的PAH分解代谢途径。PAM 1利用苜蓿根系分泌物中的某些成分作为营养物质，促进植物生长。苜蓿分枝杆菌的合作伙伴的使用是有吸引力的，以加强多环芳烃污染土壤的植物修复。
This research was focused on the isolation and characterization of a PAH-catabolizing mycobacterial strain from the petroleum hydrocarbon-contaminated rhizosphere of alfalfa, as well as on revealing some points of interaction between the microorganism and the plant. Mycolicibacterium sp. PAM1, a pyrene degrader isolated from the niche of interest to us, can catabolize fluoranthene, anthracene, fluorene, and phenanthrene. On the basis of curves of PAM1 growth with different PAHs as the sole carbon sources and on the basis of PAH-degradation rates, we found that pollutant availability to the strain decreased in the sequence phenanthrene > fluorene > fluoranthene ~ pyrene > anthracene. For each PAH, the catabolic products were identified. PAM1 was found to have the functional genes nidA and nidB. New data modeling the 2D and 3D structures, intrinsic structural disorder, and molecular dynamics of the nidA and nidB gene products were obtained. The identified genes and intermediates of pyrene degradation indicate that PAM1 has a PAH catabolic pathway that is peculiar to known mycobacterial pyrene degraders. PAM1 utilized some components of alfalfa root exudates as nutrients and promoted plant growth. The use of mycobacterial partners of alfalfa is attractive for enhancing the phytoremediation of PAH-contaminated soils.