ANALYSIS OF MIGRATORY BEHAVIOR OF NEURAL CREST AND FIBROBLASTIC CELLS IN EMBRYONIC-TISSUES
ANALYSIS OF MIGRATORY BEHAVIOR OF NEURAL CREST AND FIBROBLASTIC CELLS IN EMBRYONIC-TISSUES
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DOI：
10.1016/0012-1606(80)90462-5
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
WESTON, JA
中科院分区：
文献类型：
--
作者：
ERICKSON, CA;TOSNEY, KW;WESTON, JA
神经嵴细胞的精确迁移显然是由它们的环境控制的。确定嵴细胞正常迁移的胚胎组织间隙是否足以解释嵴细胞的分布模式，并确定其他迁移细胞是否也可以沿着这些通路沿着分布。将多种鸡和鹌鹑细胞类型移植到鸡胚胎的初始嵴细胞迁移途径中。这些细胞类型包括从培养的神经管中分离的未分化的神经嵴细胞、来自颅神经褶皱的完整嵴、嵴衍生物（色素细胞和脊神经节）、来自体节、肢芽、侧板和心室的正常胚胎成纤维细胞以及转化的成纤维细胞系（肉瘤180）。嵴细胞或其衍生物移植到嵴迁移途径都正常分布，虽然在对比的结果，当神经管原位移植，观察到较少的细胞在上皮细胞和很少或没有定位在新生的脊神经节。移植鹌鹑体节细胞有助于正常的体节结构，并没有广泛迁移在鸡宿主。其他成纤维细胞没有沿着颅或躯干嵴路径迁移，或侵入邻近组织，但在移植部位保持完整。肉瘤180细胞沿着正常躯干嵴通路分布。在肢芽或体节中异位移植的颅嵴和躯干嵴细胞及其衍生物也分散分布，并沿着腹侧迁移路径分布。移植到异位部位的成纤维细胞保持完整，不侵入宿主组织。神经嵴细胞及其衍生物在其正常通路和不熟悉的胚胎环境中具有高度运动性和侵袭性。嵴通路不单独作用于直接神经嵴细胞，因为至少1个转化细胞可以遵循嵴迁移路线。
The precise migration of neural crest cells is apparently controlled by their environment. To determine if embryonic tissue spaces in which crest cells normally migrate are sufficient to account for the pattern of crest cell distribution and to determine if other migratory cells could also distribute themselves along these pathways. A variety of chick and quail cell types were grafted into the initial crest cell migratory pathway in chicken embryos. These cell types included undifferentiated neural crest cells isolated from cultured neural tubes, intact crest from cranial neural folds, crest derivatives (pigment cells and spinal ganglia), normal embryonic fibroblastic cells from somite, limb bud, lateral plate and heart ventricle and a transformed fibroblastic cell line (Sarcoma 180). Crest cells or their derivatives grafted into the crest migratory pathway all distributed normally, although in contrast to the result when neural tubes were grafted in situ, fewer cells were observed in the epithelium and few or none were localized in the nascent spinal ganglia. Grafted quail somite cells contributed to normal somitic structures and did not migrate extensively in the chicken host. Other fibroblasts did not migrate along cranial or trunk crest pathways, or invade adjacent tissues, but remained intact at the graft site. Sarcoma 180 cells distributed themselves along the normal trunk crest pathway. Cranial and trunk crest cells and crest derivatives grafted ectopically in the limb bud or somite also dispersed, and were found along the ventral migratory pathway. Fibroblastic cells grafted into ectopic sites remained intact and did not invade host tissue. Neural crest cells and their derivatives are highly motile and invasive in their normal pathway and in unfamiliar embryonic environments. The crest pathway does not act solely to direct neural crest cells, since at least 1 transformed cell can follow the crest migratory route.