EFFECTS OF REACTION PARAMETERS ON THE INCORPORATION OF CAPRYLIC ACID INTO SOYBEAN OIL FOR PRODUCTION OF STRUCTURED LIPIDS
EFFECTS OF REACTION PARAMETERS ON THE INCORPORATION OF CAPRYLIC ACID INTO SOYBEAN OIL FOR PRODUCTION OF STRUCTURED LIPIDS
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反应参数对将辛酸掺入豆油生产结构脂质的影响
DOI：
10.1111/j.1745-4522.2006.00054.x
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发表时间：
2006
影响因子：
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通讯作者：
H. Vural
中科院分区：
文献类型：
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作者：
S. Turan;I. Karabulut;H. Vural
以大豆油（SBO）和辛酸（CA）为原料，利用sn-1,3特异性脂肪酶（tlim, Thermomyces lanuginosa）在间歇式搅拌槽反应器中不使用溶剂进行酯交换反应合成结构脂质（SLs）。考察了底物摩尔比、温度、酶载量、反应时间和搅拌速度等因素对反应的影响。为了比较不同参数对反应的影响，选择默认条件为：温度40℃，转速300 rpm，不加水，酶投加量10 wt %，反应时间6 h，底物摩尔比1:1 （SBO与CA）。采用气液色谱法对产物进行分析。底物摩尔比的增加减少了CA在SBO中的掺入。在默认反应条件下，SBO: CA摩尔比为1:7 .7时，CA掺入率最高（7.84%），摩尔比为1:4时，CA掺入率最低（0.52%）。最佳反应温度为45℃。正如预期的那样，反应中较高的TL IM脂肪酶负荷导致CA的掺入率较高。当酶用量为20 wt %时，6 h的掺入率为13.01%。300 rpm时，CA的掺入率最高（7.19%），100 rpm时，CA的掺入率最低（5.30%）。在18 h的反应中，CA掺入SBO的量急剧增加。在这个反应点之后，掺入非常缓慢。为了提高酸解的程度，用CA重复反应三次。重复反应导致三酰基甘油（TAGs）的CA含量增加。第三次反应后，tag的CA含量达到35.38%。从第一次使用酶（18.43%）到第5次使用酶（8.19%），CA的掺入率略有降低。
Structured lipids (SLs) were synthesized by transesterification of soybean oil (SBO) and caprylic acid (CA) with sn-1,3-specific lipase (TL IM, Thermomyces lanuginosa) in a batch stirred-tank reactorwithout the use of solvent. Effects of variation in substrate mole ratio, temperature, enzyme load, reaction time and mixing speeds were investigated. To compare the effects of different parameters on the reaction, the default conditions were chosen as: temperature of 40C, stirring at 300 rpm, no extra water addition, enzyme dosage of 10 wt %, reaction time of 6 h and substrate mole ratio of 1:1 (SBO to CA). Products were analyzed by gas–liquid chromatography. An increase in substrate mole ratio decreased incorporation of CA into SBO. While the highest CA incorporation (7.84%) occurred at a mole ratio of 1:0.7 (SBO: CA) at default reaction conditions, 1:4 mole ratio gave the lowest value (0.52%). Optimum reaction temperature was 45C. As expected, the higher load of TL IM lipase in the reaction led to a higher incorporation of CA. At an enzyme dosage of 20 wt % in 6 h, the incorporation was 13.01%. A high incorporation of CA (7.19%) was obtained at 300 rpm, while 100 rpm gave the lowest incorporation (5.30%). A sharp increase was obtained for incorporation of CA into SBO during 18-h reaction. After this point of the reaction, incorporation was very slow. To elevate the extent of acidolysis, reactions were repeated thrice with CA. Repeated reactions caused an increase in CA content of triacylglycerols (TAGs). After the third reaction, the CA content of TAGs reached to 35.38%. The incorporation of CA was slightly reduced from the first use (18.43%) to the fifth use of enzyme (8.19%).