Characterization and flocculating properties of a novel bioflocculant produced by Bacillus circulans
Characterization and flocculating properties of a novel bioflocculant produced by Bacillus circulans
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DOI：
10.1007/s11274-008-9943-8
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发表时间：
2009-05-01
影响因子：
4.1
通讯作者：
Li, Hua-Liang
中科院分区：
文献类型：
--
作者：
Li, Zhong;Chen, Ruo-Wei;Li, Hua-Liang
研究了一种新型生物絮凝剂PX，该絮凝剂是由环状芽孢杆菌（Bacillus circulans）X3产生的，具有优异的絮凝活性。采用乙醇沉淀、透析、凝胶渗透色谱等方法对絮凝剂进行纯化。PX的主要成分是一种酸性多糖，包括糖醛酸（19.8%）、葡糖酸（6.5%）和乙酸（0.7%）。它由半乳糖、甘露糖、木糖醇、鼠李糖和半乳糖醛酸组成，摩尔比约为5：4.1：3：2：1.2。通过GPC测定PX的分子量为约4.85 × 10（4）Da。红外光谱分析表明，絮凝剂中含有羧基、羟基、氨基和甲氧基。研究表明，该化合物在60- 100 ℃和pH 4-10范围内稳定。此外，它可以絮凝高岭土悬浮液在较宽的pH值和温度范围内的CaCl 2的存在下。
We studied a novel bioflocculant, PX, that is produced from Bacillus Bacillus circulans X3, and has excellent flocculating activity with regard to its characterization and flocculating properties. The bioflocculant was purified from supernatant by ethanol precipitation, dialysis and gel permeation chromatography (GPC). The major component of PX was an acid polysaccharide including uronic (19.8%), pyruvic (6.5%) and acetic acids (0.7%). It consisted of galactose, mannose, xylitol, rhamnose and galacturonic acid in an approximate molar ration of 5:4.1:3:2:1.2. The molecular weight of PX was about 4.85 x 10(4) Da as determined by GPC. The infrared spectrum of the bioflocculant indicated the presence of carboxyl, hydroxyl, amino and methoxyl groups. Studies of the flocculating properties revealed that it was stable at 60-100A degrees C and pH 4-10. Moreover, it could flocculate a kaolin suspension over a wide range of pH and temperature in the presence of CaCl2.