Magnetic targeting of microbubbles against physiologically relevant flow conditions.
Magnetic targeting of microbubbles against physiologically relevant flow conditions.
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DOI：
10.1098/rsfs.2015.0001
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发表时间：
2015-10-06
期刊：
影响因子：
4.4
通讯作者：
Stride E
中科院分区：
文献类型：
--
作者：
Owen J;Rademeyer P;Chung D;Cheng Q;Holroyd D;Coussios C;Friend P;Pankhurst QA;Stride E
微泡在治疗部位的定位已被证明对其在治疗应用中的有效性至关重要，例如靶向药物输送和基因治疗。为了实现定位，人们已经研究了各种不同的策略，包括生化靶向、声辐射力以及将超顺磁性纳米颗粒掺入微泡中以使其能够使用外部磁场进行操纵。这些策略中的第三种策略的优点是将微泡集中在目标区域，而不会将它们暴露在超声波下，并且可以与生化靶向结合使用，以实现更高的特异性。磁性微泡已被证明在体外和体内的治疗输送方面是有效的。然而，这项技术能否成功应用于人类仍是一个悬而未决的问题。这项研究的目的是确定可以实现靶向的流动条件的范围。体外实验结果表明，使用临床可接受的磁场可以保留磁性微泡，对于两种高剪切率(约。104 S−1)在人的小动脉和毛细血管中发现，并且高流速(约.S−3.5ml人动脉。利用猪肝脏灌流模型进一步证明了人体体内微泡滞留的可能性。
The localization of microbubbles to a treatment site has been shown to be essential to their effectiveness in therapeutic applications such as targeted drug delivery and gene therapy. A variety of different strategies for achieving localization has been investigated, including biochemical targeting, acoustic radiation force, and the incorporation of superparamagnetic nanoparticles into microbubbles to enable their manipulation using an externally applied magnetic field. The third of these strategies has the advantage of concentrating microbubbles in a target region without exposing them to ultrasound, and can be used in conjunction with biochemical targeting to achieve greater specificity. Magnetic microbubbles have been shown to be effective for therapeutic delivery in vitro and in vivo. Whether this technique can be successfully applied in humans however remains an open question. The aim of this study was to determine the range of flow conditions under which targeting could be achieved. In vitro results indicate that magnetic microbubbles can be retained using clinically acceptable magnetic fields, for both the high shear rates (approx. 104 s−1) found in human arterioles and capillaries, and the high flow rates (approx. 3.5 ml s−1) of human arteries. The potential for human in vivo microbubble retention was further demonstrated using a perfused porcine liver model.