High Performance reversed phase chromatography of Natural triglyceride mixtures: Critical pair separation
High Performance reversed phase chromatography of Natural triglyceride mixtures: Critical pair separation
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天然甘油三酯混合物的高效反相色谱：关键对分离
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
E. G. Perkins
中科院分区：
文献类型：
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作者：
A. Hamdy;E. G. Perkins
关键的甘油三酯对C48:0、C50:1、C54:3ccc和C54:3ttt以及C54:2、C52:1和C50:1的分离已经在没有任何相互作用离子（例如银）的帮助下完成。不饱和甘油三酯的理论碳数（TCN）可以根据饱和甘油三酯的碳数（CN）和容量因子（k'）关系计算出来，并用于预测关键对的分离。推导了一个数学方程，不仅可以通过碳数和双键数来识别甘油三酯，还可以识别这些甘油三酯中可能存在的酰基。具有相同碳当量 (ECN) 的每个甘油三酯类别内的甘油三酯洗脱顺序模式从双键数量最多的甘油三酯开始，到双键数量最少的甘油三酯终止，ECN 较低的甘油三酯先于 ECN 较高的甘油三酯洗脱。假设了在装有与十八烷基固定相键合的 5n 二氧化硅的高效柱上分离这些甘油三酯的可能机制。
The separation of the critical triglyceride pairs C48:0, C50:l, C54:3ccc and C54:3ttt as well as C54:2, C52:l and C50:l has been accomplished without the aid of any interacting ion such as silver. A theoretical carbon number (TCN) for the unsaturated triglycer-ides can be calculated from the carbon number (CN) and capacity factor (k’) relationship of the saturated triglycerides, and used to predict the separation of critical pairs. A mathematical equation was derived for the identification of not only the triglycerides by their carbon number and number of double bonds but also the possible acyl groups present in these triglycerides. The pattern of triglyceride elution sequence within each triglyceride category with the same equivalent carbon number (ECN) starts with the triglyceride with the highest number of double bonds and terminates with those with the lowest number of double bonds, with the lower ECN triglycer-ides eluting ahead of those with higher ECN. A possible mechanism for the separation of these triglycerides on highly efficient columns packed with 5n silica bonded with the octadecyl stationary phase is postulated.