Significance and applications of nanoparticles in siRNA delivery for cancer therapy
Significance and applications of nanoparticles in siRNA delivery for cancer therapy
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DOI：
10.1586/ecp.12.33
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发表时间：
2012-07-01
影响因子：
4.4
通讯作者：
Massaad-Massade, Liliane
中科院分区：
文献类型：
--
作者：
Ali, Hafiz Muhammad;Urbinati, Giorgia;Massaad-Massade, Liliane
RNAi是一种强大的基因沉默过程，通过使用siRNA在癌症治疗中具有很大的前景。这篇综述的目的是概述不同的siRNA递送方法，并描述这些系统的优点和缺点。siRNA的前景要比其他疗法好得多，因为它们很容易适用于任何治疗靶点。它们还承诺以精确的选择性有效地抑制基因，低至单核苷酸多态性的水平，并且可以通过在基因序列中进行筛选来容易地识别有害蛋白质或变体。利用RNAi技术治疗癌症的主要障碍是保护这种脆弱且快速代谢的生物分子，并将其有效地递送到体内的靶细胞。因此，需要用于siRNA递送的新系统，例如纳米颗粒，以帮助siRNA到达靶组织并改善其在靶组织中的生物分布。
RNAi is a powerful gene silencing process that holds great promise in cancer therapy by the use of siRNA. The aim of this review is to give an outline on different approaches to deliver siRNA and to describe the advantages and disadvantages of these systems. The prospects for siRNA are to be substantially better than other therapies, as they are easily applicable to any therapeutic target. They also promise potent gene inhibition with exquisite selectivity, down to the level of a single nucleotide polymorphism, and can easily identify offending proteins or variants by screening across a gene sequence. The main obstacle of using RNAi technology in cancer treatment is to protect such a fragile and quickly metabolized biological molecule and to efficiently deliver it in vivo to the target cells. Therefore, there is a requirement for new systems, such as nanoparticles, for siRNA delivery to help the siRNAs reach, and improve their biodistribution in, target tissues.