Genetic modification of critical enzymes and involved genes in butanol biosynthesis from biomass
Genetic modification of critical enzymes and involved genes in butanol biosynthesis from biomass
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DOI：
10.1016/j.biotechadv.2010.05.015
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发表时间：
2010-09-01
影响因子：
16
通讯作者：
Gan, Yi-Ru
中科院分区：
文献类型：
--
作者：
Huang, He;Liu, Hui;Gan, Yi-Ru
由于油价上涨以及对周围气候变化和能源危机的担忧，对生物丁醇（一种可持续的汽车燃料）的兴趣正在增加。然而，利用梭菌属的常规ABE发酵生产生物丁醇的成本高于来自当今石油化工工艺的丁醇的成本。生物丁醇的经济生产中的两个主要问题是难以控制从产酸到产溶剂的代谢转变的诱导和严重产物抑制所施加的限制。随着生物技术的发展，梭菌基因组测序的完成，基因改造是提高溶剂产率和丁醇产率的可行方法。本文重点介绍了ABE发酵途径中关键酶基因及相关基因（如编码热休克蛋白、操纵子、转录因子等基因）的过表达、插入、敲除和敲低等方面的最新研究进展。在大肠杆菌和酵母中重组这些基因的操作最近也有报道，尽管它们的丁醇产量低于梭菌。本文还对产溶剂梭菌从不同原料中生产丁醇进行了评价。(C)2010年爱思唯尔公司All rights reserved.
Interest in biobutanol, a sustainable vehicle fuel, is increasing due to rising oil prices and concerns of surrounding climate change and the energy crisis. However, the costs of biobutanol with conventional ABE fermentation by Clostridium are higher than the cost of butanol from today's petrochemical processes. Two major problems in the economic production of biobutanol are difficulty controlling the induction of a metabolic shift from acidogenesis to solventogenesis and limitations imposed by severe product inhibition. With developments in biotechnology, and the completion of genome sequencing of Clostridium, genetic modification is a viable method to improve the solvent yield and the butanol production ratio. The present article aims to highlight the latest research progress on overexpressing, inserting, knocking out, and knocking down genes of the key enzymes in the ABE fermentation pathway and other relative genes (such as genes coding for heat-shock proteins, operon, transcription, etc). Recombinant manipulations of these genes in Escherichia coli and yeast have also been reported recently, although their butanol yields are lower than in Clostridium. Butanol production with solventogenic clostridia from various feedstocks is also evaluated in this review. (C) 2010 Elsevier Inc. All rights reserved.