Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
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DOI：
10.1016/j.ijbiomac.2020.03.180
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发表时间：
2020-03
影响因子：
8.2
通讯作者：
Ying Yu;Rongqi Cui;Xin Wang;Hao Yang;Hui Li
中科院分区：
文献类型：
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作者：
Ying Yu;Rongqi Cui;Xin Wang;Hao Yang;Hui Li
聚（L-乳酸）（PLLA）是制备生物可吸收血管支架（BVS）最常用的聚合物。尽管具有显著的性能，但与永久性金属支架相比，由PLLA制成的BVS面临更高的早期血栓形成率。为了解决这个问题，我们改性PLLA膜表面与肝素模拟多糖多层膜组成的硫酸化山药多糖（SCYP）和壳聚糖（CS）通过层层（LBL）组装。利用X射线光电子能谱、扫描电子显微镜、原子力显微镜和紫外可见光谱研究了SCYP/CS多层膜的表面化学成分、形貌和生长方式。相关的血液相容性研究结果表明，多层修饰PLLA能有效地抵抗蛋白质吸附，抑制血小板粘附，延长凝血时间，防止接触和补体激活，降低溶血率。此外，多层修饰的PLLA对大肠杆菌具有良好的抗菌能力，且无细胞毒性。coliandS.金黄色葡萄球菌，以及载药/缓释行为。总之，通过沉积SCYP/CS多层膜，可以成功地获得具有血液相容性、无细胞毒性、抗菌和载药/释药行为的多功能PLLA膜，这将在血液接触生物医用材料中具有潜在的应用前景。
Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS). Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds. To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly. The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV–vis spectroscopy. The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate. Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability againstE. coliandS. aureus, and drug loading/sustained releasing behavior. Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.