Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers.
Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers.
复制标题
使用锚定二核苷酸重复引物对微卫星重复序列进行 Vectorette PCR 分离。
DOI：
--
复制
发表时间：
1996
影响因子：
14.9
通讯作者：
A. F. Markham
中科院分区：
文献类型：
--
作者：
Nicholas J. Lench;A. Norris;A. Bailey;A. Booth;A. F. Markham
我们已经开发了一种载体PCR的方法，提供一个改进的方法分离微卫星重复。修改的程序依赖于使用载体特异性引物与一组锚定二核苷酸重复引物之一组合的PCR扩增。待筛选微卫星序列的靶DNA可以来自YAC、P1、粘粒、噬菌体或质粒克隆。我们已经使用这种技术分离新的，多态性的微卫星重复克隆含有釉原蛋白基因（AMGX）位于人类染色体Xp22.3。
We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR amplification using a vectorette-specific primer in combination with one of a panel of anchored dinucleotide repeat primers. The target DNA to be screened for microsatellite sequences can be from YAC, P1, cosmid, bacteriophage or plasmid clones. We have used this technique to isolate novel, polymorphic microsatellite repeats from clones containing the amelogenin gene (AMGX) located on human chromosome Xp22.3.