Microfluidic Synthesis of Multimode Au@CoFeB-Rg3 Nanomedicines and Their Cytotoxicity and Anti-Tumor Effects
Microfluidic Synthesis of Multimode Au@CoFeB-Rg3 Nanomedicines and Their Cytotoxicity and Anti-Tumor Effects
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DOI：
10.1021/acs.chemmater.0c00797
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发表时间：
2020-06-23
影响因子：
8.6
通讯作者：
Song, Yujun
中科院分区：
文献类型：
--
作者：
Zhang, Weiwei;Zhao, Xiaoxiong;Song, Yujun
Nanomedicines (i.e., Au@CoFeB-Rg3) were devel-oped by conjugating multimode nanohybrids with active ingredients of natural herbs using Au@CoFeB nanoparticles as one model of multimode nanohybrids and the ginsenoside Rg3 as one model of active ingredients of natural herbs. Au@CoFeB nanoparticles were first synthesized using a temperature-programmed microfluidics process. Then, the surface of Au@CoFeB nanoparticles was modified via an amino-silane coupling agent of (3-aminopropyl) trimethoxysilane (APTMS) and then activated by the bifunctional amine-active cross-linker. They were thereafter conjugated to ginsenosides preactivated by APTMS by cross-linking the surface-activated nanohybrids, forming Au@CoFeB-Rg3 nanomedicines. Their multimode imaging functions were evaluated with the characterization of their magnetic and optical properties and the response to X-ray radiation. They can be optically detected via dark-field microscopy and can be imaged through X-ray computed tomography. They can also be used as magnetic resonance imaging contrast agents with excellent T2-weighted spin-echo imaging effects. Au@CoFeB-Rg3 nanomedicines exhibited distinct cytotoxicity and inhibitory effects on the proliferation of human hepatocellular carcinoma cells (HepG2/C3) and human chronic myeloid leukemia cells (K562) but were less toxic to 3T3 cells than other cells at concentrations more than 200 mu g/ mL. However, Au@CoFeB nanoparticles showed markedly lower cytotoxicity and inhibitory effects on the proliferation of these cell lines, particularly at concentrations
