Healthy effect of different proportions of marine ω-3 PUFAs EPA and DHA supplementation in Wistar rats: Lipidomic biomarkers of oxidative stress and inflammation
Healthy effect of different proportions of marine ω-3 PUFAs EPA and DHA supplementation in Wistar rats: Lipidomic biomarkers of oxidative stress and inflammation
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DOI：
10.1016/j.jnutbio.2015.07.007
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发表时间：
2015-11-01
影响因子：
5.6
通讯作者：
Medina, Isabel
中科院分区：
文献类型：
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作者：
Dasilva, Gabriel;Pazos, Manuel;Medina, Isabel
ω-3海洋脂肪酸的饮食干预可能潜在地调节与CVD、代谢综合征和癌症相关的炎症和氧化应激标志物。本研究的目的是评估不同比例的omega-3 EPA和DHA摄入量是否会引起脂质介质产生的调节，然后在使用Wistar大鼠的对照饮食动物实验中对炎症和氧化应激的不同指数产生影响。对于这种范围，应用先前开发的脂质组学SPE-LC-ESI-MS/MS方法来测定血浆样品中的脂质介质谱。将与不同比例的EPA：DHA相关的ω-3脂肪酸的效果与亚麻籽油中的ω-3 ALA和大豆油中的ω-6 LA补充剂所产生的效果进行比较。CRP在所有ω-3喂养的动物中显示出更大的炎症状态的趋势。有趣的是，比例1：1和2：1的EPA：DHA证明了显著的健康效果，产生较少的氧化环境并调节LOX和考克斯活性，以减少促炎性ARA类二十烷酸和来自EPA和DHA的氧化应激生物标志物的产生。此外，1：1和2：1鱼油饮食降低脂质介质水平的能力与降低炎症标志物（如血浆和膜中的omega-6/omega-3比值）所产生的保护作用一致。它还强调了饮食中较高DHA含量的影响，减少了炎症和氧化应激方面的健康益处。结果支持鱼油的抗氧化作用，特别是足够比例的EPA：DHA的效果。(C)2015 Elsevier Inc. All rights reserved.
Dietary intervention with omega-3 marine fatty acids may potentially modulate inflammation and oxidative stress markers related with CVD, metabolic syndrome and cancer. The aim of this study was to evaluate whether different proportions of omega-3 EPA and DHA intake provoke a modulation of the production of lipid mediators and then, an influence on different indexes of inflammation and oxidative stress in a controlled dietary animal experiment using Wistar rats. For such scope, a lipidomic SPE-LC-ESI-MS/MS approach previously developed was applied to determine lipid mediators profile in plasma samples. The effect of omega-3 fatty acids associated to different ratios EPA:DHA was compared with the effect exerted by omega-3 ALA supplementation from linseed oil and omega-6 LA from soybean oil. CRP showed a tendency to greater inflammatory status in all omega-3-fed animals. Interestingly, ratios 1:1 and 2:1 EPA:DHA evidenced a noteworthy healthy effect generating a less oxidative environment and modulating LOX and COX activities toward a decrease in the production of proinflammatory ARA eicosanoids and oxidative stress biomarkers from EPA and DHA. In addition, the ability of 1:1 and 2:1 fish oil diets to reduce lipid mediator levels was in concurrence with the protective effect exerted by decreasing inflammatory markers as omega-6/omega-3 ratio in plasma and membranes. It was also highlighted the effect of a higher DHA amount in the diet reducing the healthy benefits described in terms of inflammation and oxidative stress. Results support the antiinflammatory and antioxidative role of fish oils and, particularly, the effect of adequate proportions EPA:DHA. (C) 2015 Elsevier Inc. All rights reserved.