Barnacle in vitro Assays for Biologically Active Substances: Toxicity and Settlement Inhibition Assays Using Mass Cultured Balanus amphitrite amphitrite Darwin
Barnacle in vitro Assays for Biologically Active Substances: Toxicity and Settlement Inhibition Assays Using Mass Cultured Balanus amphitrite amphitrite Darwin
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DOI：
10.1080/08927019209386217
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发表时间：
1992-01-01
期刊：
影响因子：
2.7
通讯作者：
Bonaventura, J.
中科院分区：
文献类型：
--
作者：
Rittschof, D.;Clare, A. S.;Bonaventura, J.
开发可配制在实际涂料中的无毒或无污染的防污添加剂需要涉及目标生物体的测定。测试活性化合物的有效浓度和毒性浓度的测定是有用的。还希望该测定能够提供有关将化合物掺入不同基质中时可预期的性能的信息。这里描述的是使用藤壶（Balanus amphitrite）开发的简单实验室测定法，藤壶是一种在温带和热带海域发现的常见污垢生物。其中一项测定依赖于鲤鱼幼虫的全年同步大规模培养，其程序已被描述。实验室测定提供了藤壶毒性和沉降抑制的定量估计。所描述的方法为使用藤壶来研究测试化合物和表面的相互作用提供了一个极好的系统。
The development of non-toxic or non-polluting antifouling additives that can be formulated in practical coatings requires assays involving target organisms. Assays that test both for the effective and toxic concentrations of active compounds are useful. It is also desirable if the assay can provide information regarding the performance that can be expected if the compounds are incorporated into different matrices. Described here are the simple laboratory assays that have been developed using the barnacle, Balanus amphitrite, a common fouling organism found throughout temperate and tropical seas. One of the assays depends on synchronous year-round mass culture, the procedure for which is described, of cypris larvae. The laboratory assays provide quantitative estimates of toxicity and settlement inhibition of barnacles. The methods described provide an excellent system for the use of barnacles to study the interaction of the test compounds and surfaces.