Sucked in by eDNA – a promising tool for complementing riverine assessment of freshwater fish communities in Aotearoa New Zealand
Sucked in by eDNA – a promising tool for complementing riverine assessment of freshwater fish communities in Aotearoa New Zealand
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被 eDNA 吸引——一种很有前景的工具，可补充新西兰新西兰淡水鱼类群落的河流评估
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发表时间：
2021
期刊：
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通讯作者：
D. Gleeson
中科院分区：
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作者：
B. David;Daniel R. Fake;A. Hicks;Shaun P. Wilkinson;M. Bunce;Josh Smith;D. West;K. Collins;D. Gleeson
摘要 人们对新西兰水道健康越来越感兴趣。 2020 年国家淡水管理政策声明 (NPS-FM) 赋予资源管理者更大的责任来报告包括鱼类在内的淡水生态系统健康状况。常规鱼类监测需要大量时间，并且主要在“可涉水”的溪流区域进行。相比之下，环境 DNA (eDNA) 采样是一种相对较新且快速的技术，可以在更广泛的空间范围内检测鱼类和其他脊椎动物和无脊椎动物类群。在这项研究中，我们提供了五个可涉水溪流的数据，比较了多年标准化电捕鱼和从同一河段收集的 eDNA 样本的多样性。此外，我们还探讨了一个地点物种的 eDNA 监测“读取”数量是否可以根据一次性电捕鱼捕获提供其“近场”相对丰度的近似（半定量）指示。结果表明，虽然该方法的某些方面需要微调，但 eDNA 采样显示出在描述可涉水溪流中鱼类多样性的环境状况 (SOE) 报告方面的巨大前景。还讨论了使用水生 eDNA 监测来提供可涉水和不可涉水溪流中更广泛流域规模生物多样性（例如鸟类和外来害虫）的经济高效的指示。
ABSTRACT There is increasing interest in the health of waterways in Aotearoa New Zealand. The National Policy Statement for Freshwater Management 2020 (NPS-FM) places a greater onus on resource managers to report on the state of freshwater ecosystem health, including fish. Routine fish monitoring is time intensive and is principally conducted in ‘wadeable’ stream reaches. In comparison, environmental DNA (eDNA) sampling is a relatively recent and rapid technique that likely detects fish and other vertebrate and invertebrate taxa over a broader spatial scale. In this study, we present data for five wadeable streams comparing diversity derived from multi-year standardised electrofishing with eDNA samples collected from the same reaches. Further, we explore whether the number of eDNA monitoring ‘reads’ for species at a site may provide an approximate (semi-quantitative) indication of their ‘near field’ relative abundance based on one-pass electrofishing captures. Results indicated that while some aspects of the methodology require fine-tuning, eDNA sampling shows substantial promise for complimenting state of the environment (SOE) reporting for describing fish diversity in wadeable streams. The use of aquatic eDNA monitoring to provide a cost-effective indication of broader catchment scale biodiversity (e.g. birds and exotic pests) in wadeable and non-wadeable streams is also discussed.