SPECIALIZED CELL-TYPES IN HUMAN FETAL SMALL-INTESTINE
SPECIALIZED CELL-TYPES IN HUMAN FETAL SMALL-INTESTINE
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DOI：
10.1002/ar.1091910302
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发表时间：
1978-01-01
影响因子：
--
通讯作者：
TRIER, JS
中科院分区：
文献类型：
--
作者：
MOXEY, PC;TRIER, JS
本文报道了人胎小肠特化上皮细胞的出现时间和形态分化。应用光镜和电镜观察了36例死胎的近端和远端SB。9 ~ 10周龄时，绒毛由单层柱状上皮所覆盖，取代2-6层的复层上皮。在此过渡期间，在复层上皮的较深层中发现了独特的连接复合物和单个次级腔，并且有一些表面细胞细胞变性的证据。复层和单层柱状上皮中可见少粘和成熟的杯状细胞。隐窝形成开始近端在10-11周，在一周内，由未分化的隐窝细胞（UCC）内衬的隐窝也被确定在远端SB。这些细胞类似于成人UCC除了存在大的聚集体的糖原和大型成人型分泌颗粒（SG）的情况下，直到16周。在所有年龄段，SG都比成人小，数量也少。11-12周潘氏细胞出现，腺窝发育。与成年潘氏细胞不同，它们的SG结构不均匀，经常具有不同密度的晕核。16周时，发现细胞呈小窝状或簇状，微绒毛内有密集的微丝束，从微绒毛延伸到顶端细胞质，顶端颗粒，偶尔有小窝，微绒毛膜比相邻细胞更致密。在17周胎儿的远端SB中观察到假定的微折叠（M）细胞。这些细胞有一个不寻常的顶端边缘与不规则的突起，许多小的膜结合囊泡在细胞质中，并直接接触下面的淋巴细胞。14-15周龄的Brunner腺腺细胞与正常成人腺细胞相似。
The time of appearance and morphological differentiation of specialized epithelial cells in human fetal small intestine (SB) was described. Proximal and distal SB from 36 nonviable fetuses was studied by light microscopy and EM. During the 9- to 10-wk period, villi lined by simple columnar epithelium replaced the stratified epithelial lining which was 2-6 cell layers thick. During this transition, distinctive junctional complexes and a single secondary lumen were identified in deeper layers of stratified epithelium and there was evidence of cellular degeneration of some superficial cells. Oligomucous and mature goblet cells were present in the stratified and simple columnar epithelium. Crypt formation began proximally at 10-11 wk and, within a week, crypts lined by undifferentiated crypt cells (UCC) were also identified in the distal SB. These cells resembled adult UCC except for the presence of large aggregates of glycogen and the absence of large adult-type secretory granules (SG) until 16 wk. At all ages SG were smaller and less numerous than in adults. Paneth cells appeared with crypt development at 11-12 wk. Unlike adult Paneth cells their SG were structurally heterogeneous and frequently had cores with halos of differing density. Caveolated or tuft cells with dense bundles of microfilaments extending from microvilli into apical cytoplasm, apical granules, occasional caveolae and a microvillus membrane denser than that of adjacent cells were identified by 16 wk. Putative microfold (M) cells were seen in the distal SB of a 17 wk fetus. These cells had an unusual apical border with irregular projections, many small membrane bound vesicles in the cytoplasm and were in direct contact with underlying lymphoid cells. Glandular cells of Brunner''s glands at 14-15 wk resembled those of normal adult.