DISTINCT EFFECTS OF THROMBOSPONDIN-1 AND CISP THROMBOSPONDIN-2 ON ADRENOCORTICAL CELL SPREADING
DISTINCT EFFECTS OF THROMBOSPONDIN-1 AND CISP THROMBOSPONDIN-2 ON ADRENOCORTICAL CELL SPREADING
复制标题
DOI：
10.1016/0303-7207(94)90201-1
复制
发表时间：
1994-12-01
影响因子：
4.1
通讯作者：
FEIGE, JJ
中科院分区：
文献类型：
--
作者：
PELLERIN, S;LAFEUILLADE, B;FEIGE, JJ
促肾上腺皮质激素诱导分泌蛋白（CISP）是牛肾上腺皮质细胞响应ACTH分泌的三聚体蛋白，可能代表牛形式的血栓反应蛋白-2 （TSP2）。本研究旨在描述CISP/TSP2和TSP1（血小板反应蛋白-1）各自对肾上腺皮质细胞附着和扩散的影响。发现在血清存在的情况下，TSP1和CISP/TSP2能轻微降低肾上腺皮质细胞对塑料的附着，但对细胞扩散的影响明显不同。CISP/TSP2以剂量依赖性的方式抑制肾上腺皮质细胞的扩散（最大作用为40 μ g/ml），而TSP1（高达100 μ g/ml）对这一过程没有影响。观察CISP/TSP单独包被或CISP/TSP2与纤维连接蛋白混合包被对细胞扩散的抑制作用。我们认为CISP/TSP2对体外肾上腺皮质细胞扩散的抑制表明了该蛋白在体内肾上腺皮质细胞迁移中的作用。
Corticotropin-induced secreted protein (CISP) is a trimeric protein secreted by bovine adrenocortical cells in response to ACTH, that is likely to represent the bovine form of thrombospondin-2 (TSP2). This study was aimed at delineating the respective effects of CISP/TSP2 and TSP1 (thrombospondin-1) on adrenocortical cell attachment and spreading. TSP1 and CISP/TSP2 were found to slightly reduce the attachment of adrenocortical cells to plastic in the presence of serum but exhibited a pronounced differential effect on cell spreading. CISP/TSP2 inhibited adrenocortical cell spreading in a dose-dependent manner (maximal effect with 40 mu g/ml) whereas TSP1 (up to 100 mu g/ml) did not influence this process. The inhibition of spreading was observed whether plates were coated with CISP/TSP alone or with a mixture of CISP/TSP2 and fibronectin. We suggest that the inhibition of in vitro adrenocortical cell spreading by CISP/TSP2 is indicative of an implication of this protein in the migration of adrenocortical cells in vivo.