Inhibition of preadipocyte proliferation by mitochondrial reactive oxygen species
Inhibition of preadipocyte proliferation by mitochondrial reactive oxygen species
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DOI：
10.1016/s0014-5793(03)00862-7
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发表时间：
2003-08-28
期刊：
影响因子：
3.5
通讯作者：
Casteilla, L
中科院分区：
文献类型：
--
作者：
Carrière, A;Fernandez, Y;Casteilla, L
前脂肪细胞存在，并可增殖，以增加整个成年期的脂肪量。线粒体在这些细胞中的重要性从未被研究过，尽管我们最近报道线粒体氧化代谢在白色前脂肪细胞中是不可忽略的。线粒体活性氧的产生与呼吸链功能密切相关。越来越多的报告支持其作为信号分子的作用。本研究旨在探讨线粒体活性氧对白色前脂肪细胞增殖的影响。鱼藤酮和寡霉素，复合物I和ATP合成酶的抑制剂，分别增加过氧化氢和抑制前脂肪细胞的细胞生长（不诱导坏死或凋亡）。这些影响被部分阻止了自由基清除剂。化学解偶联剂对活性氧的产生和细胞生长有相反的影响。异丙酚，抑制复合物I，但也清除自由基，有类似的解偶联剂对这两个参数的影响。因此，线粒体活性氧可以通过影响白色前脂肪细胞池的大小来影响脂肪组织的发育。(C)2003年由Elsevier B.V.代表欧洲生物化学学会联合会出版。
Preadipocytes are present and can proliferate to increase fat mass throughout adult life. The importance of mitochondria in these cells has never been investigated, although we recently reported that mitochondrial oxidative metabolism is non-negligible in white preadipocytes. Mitochondrial reactive oxygen species generation is intimately associated with respiratory chain function. An increasing number of reports support their role as signalling molecules. The aim of this work was to study the effects of mitochondrial reactive oxygen species on proliferation of white preadipocytes. Rotenone and oligomycin, inhibitors of complex I and of ATP synthase respectively, increased H2O2 and inhibited cell growth of preadipocytes (without inducing necrosis or apoptosis). These effects were partly prevented by addition of radical scavengers. A chemical uncoupler had opposite effects on reactive oxygen species generation and cell growth. Propofol, which inhibits complex I but also scavenges free radicals, had effects similar to those of the uncoupler on both parameters. Thus, mitochondrial reactive oxygen species can influence development of adipose tissue by affecting the size of the white preadipocyte pool. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.