Three-Dimensional Inverse Opal Photonic Crystal Substrates toward Efficient Capture of Circulating Tumor Cells
Three-Dimensional Inverse Opal Photonic Crystal Substrates toward Efficient Capture of Circulating Tumor Cells
复制标题
三维反蛋白石光子晶体基底可有效捕获循环肿瘤细胞
DOI：
10.1021/acsami.7b10094
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发表时间：
2017
影响因子：
9.5
通讯作者：
Song Hongwei
中科院分区：
文献类型：
--
作者：
Xu Hongwei;Dong Biao;Xiao Qiaoqin;Sun Xueke;Zhang Xinran;Lyu Jiekai;Yang Yudan;Xu Lin;Bai Xue;Zhang Shuang;Song Hongwei
人工分形结构在循环肿瘤细胞（CTC）的检测和捕获方面引起了相当大的科学兴趣，这在癌症的诊断和预后中起着关键作用。在此，我们设计了一种仿生TiO 2反蛋白石光子晶体（IOPC）结构，将磁性Fe3O4@C6@silane纳米粒子与抗EpCAM（antiepithelial cell adhesion molecule，抗上皮细胞粘附分子）和微通道结构相结合，用于高效免疫捕获CTC。IOPC TiO 2的多孔结构和尺寸可以精确控制以模拟细胞组分，并通过与聚多巴胺（PDA）的偶联将抗EpCAM抗体进一步修饰在IOPC界面上。与平板玻璃上的CTC捕获效率相比，IOPC界面的CTC捕获效率的提高达到令人惊讶的20倍，表明IOPC是导致MCF-7细胞捕获效率显著提高的原因。孔径为415 nm的IOPC基质在1 mL/h时的最佳CTC捕获效率为92%。除了细胞亲和性，IOPC还具有光散射特性的优点，可以增强荧光标记的激发和发射光，便于实时监测CTC捕获。基于IOPC的平台在CTC捕获方面表现出优异的性能，这将朝着特异性识别疾病相关罕见细胞迈出重要一步。
Artificial fractal structures have attracted considerable scientific interest in circulating tumor cells (CTCs) detection and capture, which plays a pivotal role in the diagnosis and prognosis of cancer. Herein, we designed a bionic TiO2inverse opal photonic crystal (IOPC) structure for highly efficient immunocapture of CTCs by combination of a magnetic Fe3O4@C6@silane nanoparticles with anti-EpCAM (antiepithelial cell adhesion molecule) and microchannel structure. Porous structure and dimension of IOPC TiO2can be precisely controlled for mimicking cellular components, and anti-EpCAM antibody was further modified on IOPC interface by conjugating with polydopamine (PDA). The improvement of CTCs capture efficiency reaches a surprising factor of 20 for the IOPC interface compared to that on flat glass, suggesting that the IOPCs are responsible for the dramatic enhancement of the capture efficiency of MCF-7 cells. IOPC substrate with pore size of 415 nm leads to the optimal CTCs capture efficiency of 92% with 1 mL/h. Besides the cell affinity, IOPCs also have the advantage of light scattering property which can enhance the excitation and emission light of fluorescence labels, facilitating the real-time monitoring of CTCs capture. The IOPC-based platform demonstrates excellent performance in CTCs capture, which will take an important step toward specific recognition of disease-related rare cells.