The F-box protein family.
The F-box protein family.
复制标题
F-box蛋白家族。
DOI：
10.1186/gb-2000-1-5-reviews3002
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发表时间：
2000
期刊：
影响因子：
12.3
通讯作者：
Pagano, M
中科院分区：
文献类型：
--
作者：
Kipreos, E T;Pagano, M
F-box蛋白最初被描述为泛素连接酶复合物的组成部分，但最近发现它参与多种细胞功能，包括着丝点和翻译延伸。F-box是一个由大约50个氨基酸组成的蛋白质基序，是蛋白质与蛋白质相互作用的一个位点。F-box蛋白最初被认为是SCF泛素连接酶复合物的组成部分（以其主要成分Skp I、Cullin和F-box蛋白命名），它们结合底物进行泛素介导的蛋白水解。F-box基序通过结合核心SCF组分Skp i将F-box蛋白与SCF复合物的其他组分连接起来。最近发现F-box蛋白在非SCF蛋白复合物中发挥多种细胞功能。在出芽酵母中有11种F-box蛋白，秀丽隐杆线虫中有326种，果蝇中有22种，人类中至少有38种。F-box蛋白通常包括额外的羧基末端基序，能够进行蛋白-蛋白相互作用；酵母和人类F-box蛋白中最常见的二级基序是WD重复序列和富含亮氨酸的重复序列，它们都被发现将磷酸化的底物与SCF复合物结合。大多数F-box蛋白都有其他相关的基序，而且这些蛋白的功能还没有被明确。
F-box proteins were first described as components of ubiquitin ligase complexes, but have more recently been found to be involved in a variety of cellular functions, including in the kinetochore and in translational elongation. The F-box is a protein motif of approximately 50 amino acids that functions as a site of protein-protein interaction. F-box proteins were first characterized as components of SCF ubiquitin-ligase complexes (named after their main components, Skp I, Cullin, and an F-box protein), in which they bind substrates for ubiquitin-mediated proteolysis. The F-box motif links the F-box protein to other components of the SCF complex by binding the core SCF component Skp I. F-box proteins have more recently been discovered to function in non-SCF protein complexes in a variety of cellular functions. There are 11 F-box proteins in budding yeast, 326 predicted in Caenorhabditis elegans, 22 in Drosophila, and at least 38 in humans. F-box proteins often include additional carboxy-terminal motifs capable of protein-protein interaction; the most common secondary motifs in yeast and human F-box proteins are WD repeats and leucine-rich repeats, both of which have been found to bind phosphorylated substrates to the SCF complex. The majority of F-box proteins have other associated motifs, and the functions of most of these proteins have not yet been defined.