Roles of arabinogalactan proteins in cotyledon formation and cell wall deposition during embryo development of Arabidopsis
Roles of arabinogalactan proteins in cotyledon formation and cell wall deposition during embryo development of Arabidopsis
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阿拉伯半乳聚糖蛋白在拟南芥胚胎发育过程中子叶形成和细胞壁沉积中的作用
DOI：
10.1007/s00709-010-0204-y
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Zhao, Jie
中科院分区：
文献类型：
--
作者：
Zhong, Jing;Ren, YuJun;Yu, Miao;Ma, TengFei;Zhang, XueLian;Zhao, Jie
阿拉伯半乳聚糖蛋白（AGP）是一类高度糖基化、广泛分布于高等植物中的蛋白质。在前期研究中，我们发现拟南芥中JIM13标记的AGPs的绿色荧光主要分布在胚本身和柄基部，但在鱼雷期胚后逐渐消失。 (β-d-Glc)3 Yariv 苯基糖苷 (βGlcY) 是一种与 AGP 特异性结合的合成试剂，可以抑制胚胎发育。在这项研究中，我们通过免疫荧光、免疫酶、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）技术研究了拟南芥胚胎中AGP在胚胎萌发、子叶形成和细胞壁沉积中的功能。结果表明，50 μM βGlcY 会抑制胚胎萌发、形成异常子叶胚胎以及子叶脉管系统紊乱。与正常胚胎相比，整个异常胚胎中的AGPs和果胶信号较弱，而异常胚胎的茎尖分生组织(SAM)中的纤维素信号较强。 FTIR 分析表明，与正常胚胎相比，异常胚胎的细胞壁果胶含量相对较少，纤维素含量较高。 TEM观察发现，与正常胚胎相比，异常胚胎的SAM细胞细胞质较少，质体和淀粉粒较多，液泡较大。这些结果表明AGPs可能在拟南芥胚胎发育过程中的胚胎萌发、子叶形成、细胞壁纤维素和果胶沉积以及细胞分裂潜能中发挥作用。
Arabinogalactan proteins (AGPs) are a class of highly glycosylated, widely distributed proteins in higher plants. In the previous study, we found that the green fluorescence from JIM13-labeled AGPs was mainly distributed in embryo proper and the basal part of suspensor but gradually disappeared after the torpedo-stage embryos in Arabidopsis. And (β-d-Glc)3 Yariv phenylglycoside (βGlcY), a synthetic reagent that specifically binds to AGPs, could inhibit embryo development. In this study, as a continuous work, we investigated the AGP functions in embryo germination, cotyledon formation, and cell wall deposition in Arabidopsis embryos by using immunofluorescent, immunoenzyme, transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR) techniques. The results showed that 50 μM βGlcY caused inhibition of embryo germination, formation of abnormal cotyledon embryos, and disorder of cotyledon vasculature. Compared with the normal embryos in vitro and in vivo, the AGPs and pectin signals were quite weaker in the whole abnormal embryos, whereas the cellulose signal was stronger in the shoot apical meristem (SAM) of abnormal embryo by calcofluor white staining. The FTIR assay demonstrated that the cell wall of abnormal embryos was relatively poorer in pectins and richer in cellulose than those of normal embryos. By TEM observation, the SAM cells of the abnormal embryos had less cytoplasm, more plastid and starch grains, and larger vacuole than that of normal embryos. These results indicated that AGPs may play roles in embryo germination, cotyledon formation, cell wall cellulose and pectin deposition, and cell division potentiality during embryo development of Arabidopsis.
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影响因子：
3.1
作者：
G. Toole;M. Kačuráková;Andrew C. Smith;K. Waldron;R. Wilson
通讯作者：
G. Toole;M. Kačuráková;Andrew C. Smith;K. Waldron;R. Wilson
水稻阿拉伯半乳聚糖蛋白基因家族的全基因组鉴定、分类和表达分析（Oryza sativa L.）
影响因子：
6.9
作者：
Ma H;Zhao J
通讯作者：
Zhao J
影响因子：
1.1
作者：
R. A. Rauh;D. V. Basile
通讯作者：
R. A. Rauh;D. V. Basile
影响因子：
3.9
作者：
A. Speranza;A. Taddei;G. Gambellini;E. Ovidi;V. Scoccianti
通讯作者：
A. Speranza;A. Taddei;G. Gambellini;E. Ovidi;V. Scoccianti
影响因子：
64.8
作者：
Goldraij, A;Kondo, K;McClure, B
通讯作者：
McClure, B