Expansion of structural and functional diversities of DNA using new 5-substituted deoxyuridine derivatives by PCR with superthermophilic KOD Dash DNA polymerase

Expansion of structural and functional diversities of DNA using new 5-substituted deoxyuridine derivatives by PCR with superthermophilic KOD Dash DNA polymerase
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使用超耐热 KOD Dash DNA 聚合酶通过 PCR 使用新型 5-取代脱氧尿苷衍生物扩展 DNA 的结构和功能多样性

DOI:
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发表时间:
2001
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影响因子:
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通讯作者:
H. Ozaki
H. Ozaki
中科院分区:
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文献类型:
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作者:
H. Sawai;A. Ozaki;Fumie Satoh;T. Ohbayashi;M. M. Masud;H. Ozaki

文献摘要

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Kod DashDNA聚合酶可以通过α-亚甲基连接物接受含C5-取代基的新型脱氧尿苷衍生物的三磷酸盐,从而有效地产生相应的功能化DNA,而其他传统的DNA聚合酶不能容忍底物的修饰。
KOD Dash DNA polymerase can accept triphosphates of new deoxyuridine derivatives bearing a C5-substituent group via an α-methylene linker as a substrate in the polymerase chain reaction (PCR) yielding the corresponding functionalized DNA effectively, while other conventional DNA polymerases cannot tolerate the modification of the substrate.