Alginate-based microfluidic system for tumor spheroid formation and anticancer agent screening
Alginate-based microfluidic system for tumor spheroid formation and anticancer agent screening
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DOI：
10.1007/s10544-010-9417-2
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Cheung, Karen C.
中科院分区：
文献类型：
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作者：
Chen, Michael C. W.;Gupta, Madhuja;Cheung, Karen C.
我们展示了一种用于长期肿瘤细胞培养和药物检测的微流控系统。三维细胞培养在表征抗癌治疗中是至关重要的，因为它可以提供比肿瘤细胞的单层培养更好的模型。将乳腺肿瘤细胞包封在藻酸盐内，藻酸盐在微通道内原位凝胶化。在细胞接种后几天观察到肿瘤球体形成，并将各种浓度的阿霉素施加到包封的细胞聚集体。测量药物对细胞活力和增殖的影响。在未来，基于水凝胶的微流体装置可以包括取代目前在实验室中实施的劳动密集型筛选平台的系统的一部分，并且它们解决了改善癌细胞对抗癌药物敏感性的临床前测试的需求。
We demonstrate a microfluidic system for long-term tumor cell culture and drug testing. Three-dimensional cell culture is critical in characterizing anticancer treatments since it may provide a better model than monolayer culture of tumor cells. Breast tumor cells were encapsulated within alginate which was gelled in situ within the microchannels. Tumor spheroid formation was observed several days after cell seeding, and various concentrations of doxorubicin were applied to the encapsulated cell aggregates. Drug effects on cell viability and proliferation were measured. In future, hydrogel-based microfluidic devices can comprise part of systems which replace labor intensive screening platforms currently implemented in the laboratory, and they address a need for improving preclinical testing of cancer cell sensitivity to anti-cancer drugs.