Nanoarchitectured Superparamagnetic Iron Oxide-Doped Mesoporous Carbon Nanozymes for Glucose Sensing
Nanoarchitectured Superparamagnetic Iron Oxide-Doped Mesoporous Carbon Nanozymes for Glucose Sensing
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DOI：
10.1016/j.snb.2022.131980
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发表时间：
2022-04
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通讯作者：
M. A. Wahab;S. Hossain;M. Masud;H. Park;Aditya Ashok;M. Mustapić;Minjun Kim;D. Patel;M. Shahb
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作者：
M. A. Wahab;S. Hossain;M. Masud;H. Park;Aditya Ashok;M. Mustapić;Minjun Kim;D. Patel;M. Shahb
将nanoarchitectonics纳入nanozyme的开发，以实现目标特定的几何形状，密集的活性位点，级联催化是非常需要开发超灵敏的生物测定。金属活性位点在具有高表面积的三维（3D）中孔碳载体（MC）上的改善的分散和均匀分布可以导致增强的底物结合、迁移率和碰撞概率，并因此导致增加的过氧化物酶模拟活性。在此，我们报道了在介孔碳（IO-MC）载体上制备分散良好的超顺磁性氧化铁（IO）纳米颗粒（NPs），其具有高Fe 3+活性位点，高表面积和有序的介孔孔道，在室温下显示出有前途的催化活性。所制备的IO-MC在室温下显示出良好的纳米酶活性，具有非常有利的米氏常数Km（0.242 mM）和快速的反应速率（0.193 × 10− 7 MS −1）。最后，我们证明了IO-MC纳米酶的生物测定的功能和优势。作为概念验证，我们开发了一种上级葡萄糖检测试剂盒，其加标样品的LOD（检测限）为2 µM。这些发现表明，均匀分散在MC上的氧化铁纳米颗粒显示出作为下一代纳米酶用于开发灵敏的生物测定的有希望的潜力。
The incorporation of nanoarchitectonics into the development of nanozymes to achieve target-specific geometry, dense active sites, and cascade catalysis is highly demanded for developing ultrasensitive bioassays. The improved dispersion and uniform distribution of metal active sites onto a three-dimensional (3D) mesoporous carbon support (MC) with a high surface area can lead to enhanced substrate binding, mobility, and collision probability and therefore, increased peroxidase mimetic activity. Herein, we report the fabrication of well-dispersed superparamagnetic iron oxide (IO) nanoparticles (NPs) on mesoporous carbon (IO-MC) support with high Fe3+active sites, high surface area, and ordered mesoporous pore channels that show promising catalytic activity at room temperature. The as-prepared IO-MC shows good nanozyme activity at room temperature with highly favorable Michaelis-Menten constant,Km(0.242 mM) and fast reaction rate (0.193 × 10−7MS−1). Finally, we demonstrate the functionality and pre-eminence of IO-MC nanozyme for bioassay. As a proof-of-concept, we develop a superior glucose assay that provides a LOD (limit of detection) of 2 µM in the spiked sample. These findings suggest that homogeneously dispersed iron oxide NPs on MC show promising potential as next-generation nanozyme for developing sensitive bioassays.