Extraction and bioactivity of polygonatum polysaccharides
Extraction and bioactivity of polygonatum polysaccharides
复制标题
DOI：
10.1016/j.ijbiomac.2012.12.010
复制
发表时间：
2013-03-01
影响因子：
8.2
通讯作者：
Zhong, Dewu
中科院分区：
文献类型：
--
作者：
Jiang, Qunguang;Lv, Yunxia;Zhong, Dewu
本研究旨在探索黄精多糖的最佳提取工艺参数及其体内保肝作用。各因素对多糖得率的影响大小顺序为：提取时间(min，A)、提取次数(Gt)、液固比(C)、提取温度(C、B)、提取次数(D)。结果表明，提取时间(min、A)和液固比(C)对提取效果的影响最为显著。最佳提取工艺条件为：提取时间120min，提取温度100℃，液固比5，提取次数4。对四氯化碳(CCI4)染毒大鼠灌胃给予黄精多糖150、300、450 mg/(kg·d)。结果表明，黄精多糖可提高大鼠终末体重、肝脏抗氧化酶(超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶)活性，降低血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)活性，降低肝脏丙二醛(MDA)水平。补充黄精多糖提取物的动物肝脏切片显示病理损伤减轻，支持黄精多糖提取物能有效降低CCl_4的毒性。结论：黄精多糖治疗对CCl_4诱导的实验性大鼠肝脏氧化损伤有保护作用。(C)2012爱思唯尔B.V.保留所有权利。
The present study is to explore the optimal extraction parameters and liver protective effect of the polygonatum polysaccharides in vivo. The order of factor effects on polysaccharides production was found to be extraction time (min, A) > ratio of solvent to solid (C) > extraction temperature (degrees C, B) > extraction number (D). The results show that the effects of extraction time (min, A) and ratio of solvent to solid (C) were more significant than those of the other factors. Optimal extraction parameters were as followings: extraction time 120 min, extraction temperature 100 degrees C, ratio of solvent to solid 5, and extraction number 4. Polygonatum polysaccharides was administered orally at doses of 150, 300 and 450 mg/(kg day) to carbon tetrachloride (CCI4)-treated rats. Results showed that administration of polygonatum polysaccharides could increase rats' final body weight, liver antioxidant enzymes activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR)), decrease serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities and liver malondialdehyde (MDA) level. The liver sections obtained from animals supplemented with polygonatum polysaccharides extract demonstrated reduced pathological damages, supporting that polygonatum polysaccharides extract could effectively decrease the toxicity of CCI4. It can be concluded that polygonatum polysaccharides treatment may prevent CCI4-induced liver oxidative injury in experimental rats. (c) 2012 Elsevier B.V. All rights reserved.