Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments.
Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments.
复制标题
海洋沉积物中地衣芽孢杆菌 MB01 产生的脂肽生物表面活性剂的表征
DOI：
10.3389/fmicb.2017.00871
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发表时间：
2017
影响因子：
5.2
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Chen Y;Liu SA;Mou H;Ma Y;Li M;Hu X
由于抗生素的过度使用，抗生素耐药性已成为世界上最严重的问题之一。抗生素耐药菌更难杀灭，治疗费用更高。研究人员已经研究了抗生素替代品，如抗菌肽和脂肽。从渤海沉积物中分离到一株产脂肽类抑菌剂的功能菌MB 01，经形态学、生理生化鉴定和16 SrDNA序列测定，确定为地衣芽孢杆菌（Bacillus licheniformis）。MB 01产生的脂肽经粗提和LH-20柱层析后，经LC-ESI-MS结构鉴定，确定为环状表面活性素同系物。[M+H]+ m/z 994、1008、1022和1036均为表面活性素同系物的特征分子量。CID分析表明，脂肽的分子结构为Rn-Glu 1-Leu/Ile 2-Leu 3-Val 4-Asp 5-Leu 6-Leu/Ile 7。该脂肽对紫外光、pH值和温度的变化具有良好的耐受性。
Antibiotic resistance has become one of the world’s most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M+H]+ m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was Rn-Glu1-Leu/Ile2-Leu3-Val4-Asp5-Leu6-Leu/Ile7. The lipopeptides showed well resistance to UV light and the change of pH and temperature.