In vitro study on antioxidant potential of various drugs used in the perioperative period
In vitro study on antioxidant potential of various drugs used in the perioperative period
复制标题
DOI：
10.1111/j.1399-6576.1998.tb05073.x
复制
发表时间：
1998-01-01
影响因子：
2.1
通讯作者：
Manabe, M
中科院分区：
文献类型：
--
作者：
Kang, MY;Tsuchiya, M;Manabe, M
背景资料：由于手术创伤不仅通过产生活性氧（ROS）来加剧氧化应激，而且还削弱了针对ROS攻击的生物防御系统，因此在围手术期使用的药物的抗氧化活性可能使患者受损的氧化还原状态正常化，具有根本的重要性和巨大的临床意义。我们已经应用了基于藻红蛋白荧光的测定，其中2，2 '-偶氮双（2-脒基丙烷）二盐酸盐（AAPH）产生的过氧自由基攻击B-藻红蛋白（B-PE），导致其荧光强度线性下降;结果：根据对B-PE荧光衰减的保护作用，将药物的抗氧化活性分为3类：组I药物，其仅减慢B-PE荧光衰减（尼卡地平、维拉帕米、地尔硫卓、麻黄碱、氨茶碱、维库溴铵、利多卡因、甲哌卡因、咪达唑仑、甲硫苯丙胺、氟哌利多、氯胺酮、羟嗪、布托啡诺、泼尼松龙、氢化可的松、倍他米松、地塞米松，第二组药物，完全保护B-PE氧化并在一定时间内停止荧光衰减（多巴胺、肾上腺素、去甲肾上腺素、多巴酚丁胺、异丙肾上腺素和丁丙诺啡）;以及第III组药物，其对B-PE氧化没有保护作用（硝酸甘油、前列腺素E-1、新斯的明、泮库溴铵、琥珀胆碱、阿托品、布比卡因、喷他佐辛和肝素）。这些结果表明，组I和II药物发挥一定的抗氧化活性在体外，作为衡量他们的保护荧光衰减的B-PE。仔细考虑这些特性可能有助于促进更有效的药物应用。(C)斯堪的纳维亚麻醉学学报42（1998年）。
Background: Since surgical trauma not only intensifies the oxidative stress by generating reactive oxygen species (ROS), but also weakens the biological defense system against ROS attack, the antioxidant activity of drugs used during the perioperative period, which possibly normalizes the Impaired redox state in the patient, is of fundamental importance and great clinical interest.Methods: We have applied the phycoerythrin fluorescence-based assay, in which 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-generated peroxyl radical attacks B-phycoerythrin (B-PE) to lead to a sensitive decrease in its fluorescence intensity linearly; to evaluate the antioxidant activity of major drugs in anesthetic practice.Results: By the protective effect on B-PE fluorescence decay, the antioxidant activities of the drugs were classified into three groups: Group I drugs, which only slowed B-PE fluorescence decay (nicardipine, verapamil, diltiazem, ephedrine, aminophylline, vecuronium, Lidocaine, mepivacaine, midazolam, thiamylal, droperidol, ketamine, hydroxyzine, butorphanol, prednisolone, hydrocortisone, betamethasone, dexamethasone, methylprednisolone, and furosemide); Group II drugs, which protected B-PE oxidation completely and stopped fluorescence decay in a certain duration (dopamine, epinephrine, norepinephrine, dobutamine, isoproterenol, and buprenorphine); and Group III drugs, which had no protective effect on B-PE oxidation (nitroglycerin, prostaglandin E-1, neostigmine, pancuronium, suxamethonium, atropine, bupivacaine, pentazocine, and heparin).Conclusion: These results indicate that Group I and II drugs exert some antioxidant activity in vitro, as measured by their protection of fluorescence decay of B-PE. Careful consideration of these properties might, then, serve to facilitate more efficient drug application. (C) Acta Anaesthesiologica Scandinavica 42 (1998).